Surface-Enhanced Raman Scattering of Dopamine on Self-Assembled Gold Nanoparticles

被引:46
作者
An, Jeung-Hee [1 ]
El-Said, Waleed Ahmed [2 ]
Yea, Cheol-Heon [1 ]
Kim, Tae-Hyung [2 ]
Choi, Jeong-Woo [1 ,2 ]
机构
[1] Sogang Univ, Interdisciplinary Program & Integrated Biotechnol, Seoul 121742, South Korea
[2] Sogang Univ, Dept Chem & Biomol Engn, Seoul 121742, South Korea
关键词
Surface-Enhanced Raman Spectroscopy; Dopamine; Gold Nanoparticles; Anti-Dopamine; Immunosensor; NEUROTRANSMITTERS; SPECTROSCOPY; COMPOSITE; RELEASE; IMMUNOASSAY; FABRICATION; MOLECULES; ELECTRODE; CELLS;
D O I
10.1166/jnn.2011.3688
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Dopamine, a potent neurotransmitter in the brain, influences a variety of motivated behaviors and plays a major role in Parkinson's disease. In this study, the Raman signal of dopamine was detected on a fabricated nanoparticle-immobilized glass surface by surface-enhanced raman spectroscopy (SERS). Amine-modified glass was prepared by the self-assembly of amine-terminated silane on substrate, followed by the deposition of gold nanoparticles. The gold nanoparticles deposited on the glass surface were functionalized by anti-dopamine or dopamine. The antigen-dopamine was captured by antibody-assembled gold substrate and detected by SERS. The optical properties and morpology of the glass substrate with immobilized gold nanoparticles were analyzed by scanning electron microscopy and UV-VIS absorption spectroscopy. The Raman spectrum of dopamine displayed broad bands at 1267, 1331, 1158, 1478, 1578 and 1584 cm(-1). The strongest peaks in the spectra (at 1267 and 1478 cm(-1)) were identified as phenolic carbon-oxygen and phenyl C=C stretches, respectively. A working curve of the SERS signal constructed from cathecol ring vibration versus antigen-dopamine concentration was obtained at 1478 cm(-1), and the non-optimized detection limit for anti-dopamine surface antigen was as low as 1 ng/ml. These results suggest that SERS-based immunosensor can be a promising tool for the detection and screening of neurotransmitters.
引用
收藏
页码:4424 / 4429
页数:6
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