Genetically encoded single circularly permuted fluorescent protein-based intensity indicators

被引：13

作者：

Liu, Wenfeng ^{[1
]}

Deng, Mengying ^{[1
]}

Yang, Chengming ^{[2
]}

Liu, Feng ^{[1
]}

Guan, Xinmeng ^{[1
]}

Du, Yichen ^{[3
]}

Wang, Liang ^{[1
]}

Chu, Jun ^{[1
,4
]}

机构：

[1] Chinese Acad Sci, Shenzhen Inst Adv Technol, Lab Biomed Opt & Mol Imaging, Shenzhen 518055, Peoples R China

[2] Southern Univ Sci & Technol Hosp, Dept Ophthalmol, Shenzhen 518055, Peoples R China

[3] Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Peoples R China

[4] Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen Hong Kong Inst Brain Sci, CAS Key Lab Hlth Informat,Shenzhen Inst Synthet B, Shenzhen 518055, Peoples R China

来源：

JOURNAL OF PHYSICS D-APPLIED PHYSICS | 2020年 / 53卷 / 11期

基金：

中国国家自然科学基金;

关键词：

fluorescent protein; circular permutation; cpFP; genetically encoded indicator; GCaMP; IN-VIVO; CALCIUM INDICATORS; NEURAL CIRCUITS; LIVING CELLS; GREEN; PROBE; SENSORS; REDOX; CA2+; BRIGHT;

D O I：

10.1088/1361-6463/ab5dd8

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

Fluorescent protein (FP)-based genetically encoded indicators are valuable tools for reporting signaling activities with high spatial-temporal resolution in living cells and animal models. Thereinto, single circularly permuted FP-based intensity sensors (cpFP sensors or cpFP-based sensors, unless otherwise specified) are of particular interest due to their unprecedented high sensitivity and fast kinetics. In this review, we summarized the recent progress with cpFP sensors, focusing on the practical considerations in sensor development and usage, and categorization of cpFP sensors. We also discuss future directions in improvement of existing sensors, development of new sensors, and functional super-resolution imaging with cpFP sensors.

引用

页数：22

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