Prosthechea karwinskii, an orchid used as traditional medicine, exerts anti-inflammatory activity and inhibits ROS

被引:22
|
作者
Soledad Barragan-Zarate, Gabriela [1 ]
Lagunez-Rivera, Luicita [1 ]
Solano, Rodolfo [1 ]
Arlen Pineda-Pena, Elizabeth [2 ]
Yolia Landa-Juarez, Arizai [2 ]
Evangelina Chavez-Pina, Aracely [2 ]
Carranza-Alvarez, Candy [3 ]
Manuel Hernandez-Benavides, Diego [3 ]
机构
[1] Inst Politecn Nacl, Ctr Interdisciplinario Invest Desarrollo Integral, Unidad Oaxaca, Hornos 1003, Xoxocotlan 71230, Oaxaca, Mexico
[2] Inst Politecn Nacl, Lab Farmacol, Escuela Nacl Med & Homeopatia, Guillermo Massieu Helguera 239, Ciudad De Mexico 07320, Mexico
[3] Univ Autonoma San Luis Potosi, Unidad Acad Multidisciplinaria Zona Huasteca, Romualdo del Campo 501, Ciudad Valles 79060, San Luis Potosi, Mexico
关键词
Oxidative stress; Anti-inflammatory; Gastroprotection; DCFH-DA; Orchidaceae; UPLC-ESI-qTOF-MS/MS; INDUCED GASTRIC INJURY; OXIDATIVE STRESS; DOCOSAHEXAENOIC ACID; ANTIOXIDANT ACTIVITIES; AQUEOUS EXTRACT; INFLAMMATION; EMBELIN; PARTICIPATION; DICLOFENAC; DECREASES;
D O I
10.1016/j.jep.2020.112632
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Ethnopharmacological relevance: Prosthechea karwinskii (Mart) J.M.H. Shaw is a Mexican orchid used in traditional medicine by some indigenous communities to treat issues related to inflammation (cough, wounds, burns, and diabetes). Pharmacological research of this orchid could validate its therapeutic uses and demonstrate its potential for treating other health conditions of high prevalence in Mexico, including those associated with oxidative stress such as diabetes, cancer, atherosclerosis, and hypertension as well as inflammation. Aim of the study: The leaf extract from P. karwinskii was examined to identify its compounds and elucidate its inhibitory effect on reactive oxygen species as well as its anti-inflammatory activity and gastroprotective effects in an animal model. Materials and methods: Compounds were identified via ultra-high-performance liquid chromatography coupled with electrospray ionization with quadrupole time of flight-mass spectrometry. Inhibition of reactive oxygen species was determined ex vivo in peripheral blood mononuclear cells with 2',7'-dichlorodihydrofluorescein diacetate. The anti-inflammatory activity was assessed using a carrageenan-induced paw edema model in Wistar rats; nitric oxide and tumor necrosis factor alpha levels were quantified. The gastroprotective effect was evaluated in Wistar rats with indomethacin-induced gastric injury. Results: Nine compounds were identified in the P. karwinskii leaf extract. Most compounds, such as quinic acid, malic acid, neochlorogenic acid, chlorogenic acid, rutin, embelin, pinellic acid, and azelaic acid, were reported to exhibit antioxidant and/or anti-inflammatory activity. The extract was also found to inhibit reactive oxygen species in the ex vivo model. Unlike other anti-inflammatory drugs, the extract exerted a dual effect anti-inflammatory activity and protection of the gastric mucosa. The results showed that the extract could significantly inhibit the release of nitric oxide without a dose-response relationship. Conclusion: P. karwinskii leaf extract inhibited reactive oxygen species and exerted an anti-inflammatory effect. Moreover, this extract did not induce gastric damage in the animals. The bioactivity of the species was found to support its use in traditional medicine. This orchid could be used to treat inflammatory diseases without causing the side effects associated with nonsteroidal anti-inflammatory drugs. It can also be employed to treat other pathological conditions associated with oxidative stress. The findings herein form the basis for the future discovery of natural products that may serve as safe alternative therapies for inflammatory disorders.
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页数:9
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