Liquid Crystal Ordering and Isotropic Gelation in Solutions of Four-Base-Long DNA Oligomers

被引：53

作者：

Fraccia, Tommaso P. ^{[1
,5
]}

Smith, Gregory P. ^{[2
,3
]}

Bethge, Lucas ^{[4
]}

Zanchetta, Giuliano ^{[1
]}

Nava, Giovanni ^{[1
]}

Klussmann, Sven ^{[4
]}

Clark, Noel A. ^{[2
,3
]}

Bellini, Tommaso ^{[1
]}

机构：

[1] Univ Milan, Dipartimento Biotecnol Med & Med Traslaz, Via Fratelli Cervi 93, I-20090 Milan, Italy

[2] Univ Colorado, Dept Phys, Boulder, CO 80309 USA

[3] Univ Colorado, Liquid Crystal Mat Res Ctr, Boulder, CO 80309 USA

[4] NOXXON Pharma AG, Max Dohrn Str 8-10, D-10589 Berlin, Germany

[5] Univ San Raffaele Roma, Dipartimento Sci Umane & Promoz Qualita Vita, Via Val Cannuta 247, I-00166 Rome, Italy

来源：

ACS NANO | 2016年 / 10卷 / 09期

基金：

美国国家科学基金会;

关键词：

liquid crystals; self-assembly; DNA nanotechnology; origin of life; coaxial stacking of DNA; ABIOTIC LIGATION; NEMATIC PHASES; CRYSTALLIZATION; STACKING; POLYMERS; DYNAMICS; CHAINS;

D O I：

10.1021/acsnano.6b03622

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Liquid crystal ordering is reported in aqueous solutions of the oligomer 5'-ATTAp-3' and of the oligomer 5'-GCCGp-3'. In both systems, we quantitatively interpret ordering as stemming from the chaining of molecules via a "running-bond" type of pairing, a self assembly process distinct from the duplex aggregation previously reported for longer oligonucleotides. While concentrated solutions of 5'-ATTAp-3' show only a columnar liquid crystal phase, solutions of 5'-GCCGp-3' display a rich phase diagram, featuring a chiral nematic phase analogous to those observed in solutions of longer oligonucleotides and two unconventional phases, a columnar crystal and, at high concentration, an isotropic amorphous gel. The appearance of these phases, which can be interpreted on the basis of features of 5'-GCCGp-3'molecular structure, suggests distinctive assembly motifs specific to ultrashort oligonucleotides.

引用

页码：8508 / 8516

页数：9

共 31 条

[1] Liquid crystal self-assembly of random-sequence DNA oligomers [J].