Identification of glyoxalase-I as a protein marker in a mouse model of extremes in trait anxiety

被引：209

作者：

Krömer, SA ^{[1
]}

Kessler, MS ^{[1
]}

Milfay, D ^{[1
]}

Birg, IN ^{[1
]}

Bunck, M ^{[1
]}

Czibere, L ^{[1
]}

Panhuysen, M ^{[1
]}

Pütz, B ^{[1
]}

Deussing, JM ^{[1
]}

Holsboer, F ^{[1
]}

Landgraf, R ^{[1
]}

Turck, CW ^{[1
]}

机构：

[1] Max Planck Inst Psychiat, D-80804 Munich, Germany

来源：

JOURNAL OF NEUROSCIENCE | 2005年 / 25卷 / 17期

关键词：

anxiety; enzyme; biomarker; proteomics; HAB; depression; animal model; microarray;

D O I：

10.1523/JNEUROSCI.0115-05.2005

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

For > 15 generations, CD1 mice have been selectively and bidirectionally bred for either high-anxiety-related behavior (HAB-M) or low-anxiety-related behavior (LAB-M) on the elevated plus-maze. Independent of gender, HAB-M were more anxious than LAB-M animals in a variety of additional tests, including those reflecting risk assessment behaviors and ultrasound vocalization, with unselected CD1 "normal" control (NAB-M) and cross-mated (CM-M) mice displaying intermediate behavioral scores in most cases. Furthermore, in both the forced-swim and tail-suspension tests, LAB-M animals showed lower scores of immobility than did HAB-M and NAB-M animals, indicative of a reduced depression-like behavior. Using proteomic and microarray analyses, glyoxalase-I was identified as a protein marker, which is consistently expressed to a higher extent in LAB-M than in HAB-M mice in several brain areas. The same phenotype-dependent difference was found in red blood cells with NAB-M and CM-M animals showing intermediate expression profiles of glyoxalase-I. Additional studies will examine whether glyoxalase-I has an impact beyond that of a biomarker to predict the genetic predisposition to anxiety- and depression-like behavior.

引用

页码：4375 / 4384

页数：10

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[1] Intra- and interstrain differences in models of "behavioral despair" [J].