Towards monitoring real-time cellular response using an integrated microfluidics-matrix assisted laser desorption ionisation/nanoelectrospray ionisation-ion mobility-mass spectrometry platform

被引：21

作者：

Enders, J. R. ^{[1
,2
,3
]}

Marasco, C. C. ^{[3
,4
]}

Kole, A. ^{[3
]}

Nguyen, B. ^{[3
]}

Sevugarajan, S. ^{[1
,2
]}

Seale, K. T. ^{[3
,4
]}

Wikswo, J. P. ^{[3
,4
,5
,6
]}

McLean, J. A. ^{[1
,2
,3
]}

机构：

[1] Vanderbilt Univ, Dept Chem, Nashville, TN 37235 USA

[2] Vanderbilt Univ, Vanderbilt Inst Chem Biol, Nashville, TN 37235 USA

[3] Vanderbilt Univ, Vanderbilt Inst Integrat Biosyst Res & Educ, Nashville, TN 37235 USA

[4] Vanderbilt Univ, Dept Biomed Engn, Nashville, TN 37235 USA

[5] Vanderbilt Univ, Dept Phys & Astron, Nashville, TN 37235 USA

[6] Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37235 USA

来源：

IET SYSTEMS BIOLOGY | 2010年 / 4卷 / 06期

关键词：

SUB-NANOLITER VOLUMES; HUMAN PLASMA PROTEOME; ELECTROSPRAY-IONIZATION; CAPILLARY-ELECTROPHORESIS; ACIDIFICATION RATE; SYSTEMS BIOLOGY; PH MEASUREMENTS; IN-VIVO; CHIP; DEVICES;

D O I：

10.1049/iet-syb.2010.0012

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The combination of microfluidic cell trapping devices with ion mobility-mass spectrometry offers the potential for elucidating in real time the dynamic responses of small populations of cells to paracrine signals, changes in metabolite levels and delivery of drugs and toxins. Preliminary experiments examining peptides in methanol and recording the interactions of yeast and Jurkat cells with their superfusate have identified instrumental set-up and control parameters and online desalting procedures. Numerous initial experiments demonstrate and validate this new instrumental platform. Future outlooks and potential applications are addressed, specifically how this instrumentation may be used for fully automated systems biology studies of the significantly interdependent, dynamic internal workings of cellular metabolic and signalling pathways.

引用

页码：416 / 427

页数：12

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