Phytohemagglutinin inhibits lymphoid tumor growth in vitro and in vivo

被引:9
作者
D'Costa, SS
Hurwitz, JL
机构
[1] St Jude Childrens Res Hosp, Dept Immunol, Memphis, TN 38105 USA
[2] Univ Tennessee, Dept Pathol, Memphis, TN USA
关键词
phytohemagglutinin; lymphoid tumors; apoptosis; cell growth inhibition; PHA;
D O I
10.1080/1042819031000119217
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Cure rates for a variety of leukemias and lymphomas have improved dramatically over the past several decades, but relapsed disease continues to account for thousands of deaths per year. Viable treatment options for relapsed disease are few, encouraging the development of novel therapies. In the present paper, we describe phytohemagglutinin (PHA), a standard T cell mitogen, as an inhibitor of both T- and B-cell tumors. In vitro studies show that PHA can inhibit incorporation of H-3-thymidine and mediate apoptosis of B- and T-cell tumor lines. The inhibitory effects are enhanced when PHA is used in conjunction with the cell cycle directed drug 5-fluorouracil (5-FU). Phytohemagglutinin treatments can also impede tumor growth in mice while showing no toxic side effects in this animal model.
引用
收藏
页码:1785 / 1791
页数:7
相关论文
共 27 条
[1]   Generation of heterohybridomas secreting human immunoglobulins; pokeweed mitogen prestimulation is highly effective but phytohemagglutinin drives most B cells into apoptosis [J].
Arinbjarnarson, S ;
Valdimarsson, H .
JOURNAL OF IMMUNOLOGICAL METHODS, 2002, 259 (1-2) :139-148
[2]   Biological and therapeutic aspects of infant leukemia [J].
Biondi, A ;
Cimino, G ;
Pieters, R ;
Pui, CH .
BLOOD, 2000, 96 (01) :24-33
[3]   Activation of T-cells through an antigen-independent alternative pathway induces precocious sensitivity to Fas-induced apoptosis [J].
Bonay, M ;
Bouchonnet, F ;
Lecossier, D ;
Boumsell, L ;
Soler, P ;
Grodet, A ;
Robertson, MJ ;
Hance, AJ .
IMMUNOLOGY LETTERS, 1997, 59 (02) :107-113
[4]   Customized mitogen or antibody treatments enhance the sensitivity of lymphoid tumors to 5-fluorouracil in vitro and in vivo [J].
D'Costa, S ;
Hurwitz, JL .
LEUKEMIA & LYMPHOMA, 2003, 44 (05) :841-847
[5]   Red blood cells inhibit activation-induced cell death and oxidative stress in human peripheral blood T lymphocytes [J].
Fonseca, AM ;
Porto, G ;
Uchida, K ;
Arosa, FA .
BLOOD, 2001, 97 (10) :3152-3160
[6]  
GERMAIN RN, 1997, CURR BIOL, V7, P640
[7]  
HOUGHTON JA, 1980, CANCER, V45, P1159, DOI 10.1002/1097-0142(19800315)45:5+<1159::AID-CNCR2820451320>3.0.CO
[8]  
2-Z
[9]   ANTIGEN-INDUCIBLE, H-2-RESTRICTED, INTERLEUKIN-2-PRODUCING T-CELL HYBRIDOMAS - LACK OF INDEPENDENT ANTIGEN AND H-2 RECOGNITION [J].
KAPPLER, JW ;
SKIDMORE, B ;
WHITE, J ;
MARRACK, P .
JOURNAL OF EXPERIMENTAL MEDICINE, 1981, 153 (05) :1198-1214
[10]   SUPERANTIGENS AND THEIR POTENTIAL ROLE IN HUMAN-DISEASE [J].
KOTZIN, BL ;
LEUNG, DYM ;
KAPPLER, J ;
MARRACK, P .
ADVANCES IN IMMUNOLOGY, VOL 54, 1993, 54 :99-166