Tobacco microtubule-associated protein, MAP65-1c, bundles and stabilizes microtubules

被引：13

作者：

Meng, Qiutao ^{[1
]}

Du, Jizhou ^{[1
]}

Li, Jiejie ^{[1
]}

Lue, Xiaomei ^{[1
]}

Zeng, Xian ^{[1
]}

Yuan, Ming ^{[1
]}

Mao, Tonglin ^{[1
]}

机构：

[1] China Agr Univ, Coll Biol Sci, Dept Plant Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China

来源：

PLANT MOLECULAR BIOLOGY | 2010年 / 74卷 / 06期

基金：

中国国家自然科学基金;

关键词：

NtMAP65-1c; Microtubules-associated protein; Microtubule stabilization; Katanin; DYNAMICS; BINDING; CYTOSKELETON; ATMAP65-1; TUBULIN;

D O I：

10.1007/s11103-010-9694-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three genes that encode MAP65-1 family proteins have been identified in the Nicotiana tabacum genome. In this study, NtMAP65-1c fusion protein was shown to bind and bundle microtubules (MTs). Further in vitro investigations demonstrated that NtMAP65-1c not only alters MT assembly and nucleation, but also exhibits high MT stabilizing activity against cold or katanin-induced destabilization. Analysis of NtMAP65-1c-GFP expressing BY-2 cells clearly demonstrated that NtMAP65-1c was able to bind to MTs during specific stages of the cell cycle. Furthermore, in vivo, NtMAP65-1c-GFP-bound cortical MTs displayed an increase in resistance against the MT-disrupting drug, propyzamide, as well as against cold temperatures. Taken together, these results strongly suggest that NtMAP65-1c stabilizes MTs and is involved in the regulation of MT organization and cellular dynamics.

引用

页码：537 / 547

页数：11

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