Production and purification of VP2 protein of porcine parvovirus expressed in an insect-baculovirus cell system

被引：20

作者：

Zhou, Hongchao ^{[2
]}

Yao, Guizhe ^{[1
]}

Cui, Shangjin ^{[1
]}

机构：

[1] Harbin Vet Res Inst CAAS, State Key Lab Vet Biotechnol, Div Swine Infect Dis, Harbin 150001, Peoples R China

[2] NW A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China

来源：

VIROLOGY JOURNAL | 2010年 / 7卷

关键词：

ANTIGEN CARRIER; VIRUS; PARTICLES; VACCINATION; CANINE;

D O I：

10.1186/1743-422X-7-366

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The porcine parvovirus (PPV) VP2 protein was expressed in an insect-baculovirus cell system and was purified using Ni-NTA affinity column chromatography. The recombinant 6-His-tagged VP2 protein with molecular mass (Mr) of about 64 kDa was detected by anti-his antibody and anti-PPV serum. Electron microscopy showed that the purified VP2 protein assembled into spherical particles with diameters ranging from 20 to 22 nm. The expressed VP2 was antigenically similar to the native capsid protein according to HA and a Western blotting assay performed with polyclonal antibodies collected from an outbreak of PPV in one farm. This study provides a foundation for the application of VP2 protein in the clinical diagnosis of PPV or in the vaccination against PPV in the future.

引用

页数：6

共 25 条

[1] Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus [J].

Allan, GM ;

Kennedy, S ;

McNeilly, F ;

Foster, JC ;

Ellis, JA ;

Krakowka, SJ ;

Meehan, BM ;

Adair, BM .

JOURNAL OF COMPARATIVE PATHOLOGY, 1999, 121 (01) :1-11

[2] A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure [J].

Antonis, Adriaan F. G. ;

Bruschke, Christianne J. M. ;

Rueda, Paloma ;

Maranga, Luis ;

Casal, J. Ignacio ;

Vela, Carmen ;

Hilgers, Luuk A. Th. ;

Belt, Peter B. G. M. ;

Weerdmeester, Klaas ;

Carrondo, Manuel J. T. ;

Langeveld, Jan P. M. .

VACCINE, 2006, 24 (26) :5481-5490

[3] Genome organization of the Kresse strain of porcine parvovirus: Identification of the allotropic determinant and comparison with those of NADL-2 and field isolates [J].

Bergeron, J ;

Hebert, B ;

Tijssen, P .

JOURNAL OF VIROLOGY, 1996, 70 (04) :2508-2515

[4] Parvovirus diagnostics and vaccine production in insect cells [J].

Casal, JI .

CYTOTECHNOLOGY, 1996, 20 (1-3) :261-270

[5]

Casal JI, 1999, BIOTECHNOL APPL BIOC, V29, P141

[6]

Gardner IA, 1996, J AM VET MED ASSOC, V208, P863

[7]

Hu YC, 1999, BIOTECHNOL BIOENG, V63, P721, DOI 10.1002/(SICI)1097-0290(19990620)63:6<721::AID-BIT10>3.0.CO

[8]

2-O

[9] Mapping the antigenic structure of porcine parvovirus at the level of peptides [J].

Kamstrup, S ;

Langeveld, J ;

Botner, A ;

Nielsen, J ;

Schaaper, WMM ;

Boshuizen, RS ;

Casal, JI ;

Hojrup, P ;

Vela, C ;

Meloen, R ;

Dalsgaard, K .

VIRUS RESEARCH, 1998, 53 (02) :163-173

[10] Viral wasting syndrome of swine: Experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus [J].

Krakowka, S ;

Ellis, JA ;

Meehan, B ;

Kennedy, S ;

McNeilly, F ;

Allan, G .

VETERINARY PATHOLOGY, 2000, 37 (03) :254-263

← 1 2 3 →