Effects of ZnO nanoparticles and its bulk form on growth, antioxidant defense system and expression of oxidative stress related genes in Hordeum vulgare L

被引:52
作者
Azarin, Kirill [1 ]
Usatov, Alexander [1 ]
Minkina, Tatiana [1 ]
Plotnikov, Andrey [1 ]
Kasyanova, Alexandra [1 ]
Fedorenko, Aleksei [1 ]
Duplii, Nadezhda [1 ]
Vechkanov, Evgeniy [1 ]
Rajput, Vishnu D. [1 ]
Mandzhieva, Saglara [1 ]
Alamri, Saud [2 ]
机构
[1] Southern Fed Univ, Rostov Na Donu 344090, Russia
[2] King Saud Univ, Coll Sci, Dept Bot & Microbiol, Riyadh 11451, Saudi Arabia
关键词
Antioxidant enzymes; Gene expression; Nanoparticles; Zinc; Barley; ZINC-OXIDE NANOPARTICLES; COMPARATIVE PHYTOTOXICITY; ANTIBACTERIAL ACTIVITY; SUPEROXIDE-DISMUTASE; HYDROGEN-PEROXIDE; HEAVY-METALS; TOXICITY; ENZYMES; TOMATO; PLANTS;
D O I
10.1016/j.chemosphere.2021.132167
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A comparative analysis of physio-biochemical indices and transcriptional activity of oxidative stress genes in barley (Hordeum vulgare L.) seedlings after 7-days exposure to bulk-and nano-ZnO (300 and 2000 mg/L) was carried out. A dose-dependent reduction in the length and weight of roots and shoots, as well as a significant accumulation of Zn in plant parts, was shown. Alterations in the shape and size of organelles, cytoplasmic vacuolization, and chloroplast and mitochondrial disorganization were also revealed. These processes are particularly pronounced when H. vulgare is exposed to the higher concentrations of nano-ZnO. The study of the antioxidant defense system revealed mainly an increase in the level of reduced glutathione and the activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione S-transferase (GST). The increases in activity, by 4-fold and 3-fold, was found for glutathione transferase in the roots when exposed to 2000 mg/L bulk-and nano-ZnO, respectively. The study of transcriptional activity demonstrated that in the roots under the influence of bulk-and nano-ZnO, along with Mn-SOD, Fe-SOD is highly expressed, mainly associated with the protection of chloroplasts. Analysis of the Cat 1 and Cat 2 gene expression showed that the main contribution to the increase in catalase activity in treated H. vulgare is made by the CAT-1 isozyme. Generally, in response to the impact of the studied ZnO forms, the antioxidant defense system is activated in H. vulgare, which effectively prevents the progression of oxidative stress in early stages of plant ontogenesis. Nevertheless, with constant exposure to bulk-and nano-ZnO at high concentrations, such activation leads to a depletion of the plant's energy resources, which negatively affects its growth and development. The results obtained could be useful in predicting the risks associated with the further transfer of nano-ZnO to the environment.
引用
收藏
页数:12
相关论文
共 76 条
[1]   Assessment of ZnO-NPs toxicity in maize: An integrative microRNAomic approach [J].
Adhikari, Sinchan ;
Adhikari, Ayan ;
Ghosh, Supriya ;
Roy, Doyel ;
Azahar, Ikbal ;
Basuli, Debapriya ;
Hossain, Zahed .
CHEMOSPHERE, 2020, 249
[2]   Differential responses of maize (Zea mays) at the physiological, biomolecular, and nutrient levels when cultivated in the presence of nano or bulk ZnO or CuO or Zn2+ or Cu2+ ions [J].
Ahmed, Bilal ;
Rizvi, Asfa ;
Syed, Asad ;
Elgorban, Abdallah M. ;
Khan, Mohammad Saghir ;
AL-Shwaiman, Hind A. ;
Musarrat, Javed ;
Lee, Jintae .
JOURNAL OF HAZARDOUS MATERIALS, 2021, 419
[3]   Nanoparticles in the soil-plant system: a review [J].
Ahmed, Bilal ;
Rizvi, Asfa ;
Ali, Khursheed ;
Lee, Jintae ;
Zaidi, Almas ;
Khan, Mohammad Saghir ;
Musarrat, Javed .
ENVIRONMENTAL CHEMISTRY LETTERS, 2021, 19 (02) :1545-1609
[4]   Impact of metal-oxide nanoparticles on growth, physiology and yield of tomato (Solanum lycopersicum L.) modulated by Azotobacter salinestris strain ASM [J].
Ahmed, Bilal ;
Syed, Asad ;
Rizvi, Asfa ;
Shahid, Mohammad ;
Bahkali, Ali H. ;
Khan, Mohammad Saghir ;
Musarrat, Javed .
ENVIRONMENTAL POLLUTION, 2021, 269
[5]   Understanding the phyto-interaction of heavy metal oxide bulk and nanoparticles: evaluation of seed germination, growth, bioaccumulation, and metallothionein production [J].
Ahmed, Bilal ;
Rizvi, Asfa ;
Zaidi, Almas ;
Khan, Mohammad Saghir ;
Musarrat, Javed .
RSC ADVANCES, 2019, 9 (08) :4210-4225
[6]   Toxicity assessment of metal oxide nano-pollutants on tomato (Solanum lycopersicon): A study on growth dynamics and plant cell death [J].
Ahmed, Bilal ;
Khan, Mohammad Saghir ;
Musarrat, Javed .
ENVIRONMENTAL POLLUTION, 2018, 240 :802-816
[7]   Mitochondrial and Chromosomal Damage Induced by Oxidative Stress in Zn2+ Ions, ZnO-Bulk and ZnO-NPs treated Allium cepa roots [J].
Ahmed, Bilal ;
Dwivedi, Sourabh ;
Abdin, Malik Zainul ;
Azam, Ameer ;
Al-Shaeri, Majed ;
Khan, Mohammad Saghir ;
Saquib, Quaiser ;
Al-Khedhairy, Abdulaziz A. ;
Musarrat, Javed .
SCIENTIFIC REPORTS, 2017, 7
[8]   Transformation and bioavailability of metal oxide nanoparticles in aquatic and terrestrial environments. A review [J].
Amde, Meseret ;
Liu, Jing-Fu ;
Tan, Zhi-Qiang ;
Bekana, Deribachew .
ENVIRONMENTAL POLLUTION, 2017, 230 :250-267
[9]   A review on metal-based nanoparticles and their toxicity to beneficial soil bacteria and fungi [J].
Ameen, Fuad ;
Alsamhary, Khawla ;
Alabdullatif, Jamila A. ;
ALNadhari, Saleh .
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2021, 213
[10]   Impact of zinc and zinc oxide nanoparticles on the physiological and biochemical processes in tomato and wheat [J].
Amooaghaie, Rayhaneh ;
Norouzi, Maryam ;
Saeri, Mohammad .
BOTANY, 2017, 95 (05) :441-455