Cloning, Expression, and Characterization of a GHF 11 Xylanase from Alteromonas macleodii HY35 in Escherichia coli

被引：2

作者：

Tian, Yanjie ^{[1
]}

Xu, Jia ^{[1
]}

Shi, Jianing ^{[1
]}

Kong, Mengyuan ^{[1
]}

Guo, Changjiang ^{[1
]}

Cui, Caixia ^{[1
]}

Wang, Yongtao ^{[2
]}

Wang, Yan ^{[1
]}

Zhou, Chenyan ^{[1
,3
]}

机构：

[1] Xinxiang Med Univ, Sch Life Sci & Technol, Synthet Biol Engn Lab Henan Prov, Xinxiang, Peoples R China

[2] Xinxiang Med Univ, Affiliated Hosp 1, Weihui, Peoples R China

[3] Xinxiang Med Univ, Sch Life Sci & Technol, 601 Jinsui Rd, Xinxiang 453003, Peoples R China

来源：

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 2022年 / 68卷 / 03期

关键词：

Alteromonas macleodii HY35; xylanase; glycosyl hydrolase family11; expression; enzymatic properties; GH11; XYLANASE; TOLERANT XYLANASE; MARINE; THERMOSTABILITY; PURIFICATION; STRAIN; GENE;

D O I：

10.2323/jgam.2021.10.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A xylanase gene xynZT-1 from Alteromonas macleodii HY35 was cloned and expressed in Escherichia coli (E. coli). The sequencing results showed that the ORF of xynZT-1 was 831 bp. The xylanase DNA sequence encoded a 29 amino acids (aa) signal peptide and a 247-aa mature peptide. The XynZT-1 has been a calculated molecular weight (MW) of 27.93 kDa, isoelectric point (pI) of 5.11 and the formula C1266H1829N327O384S5. The amino acid sequence of the xynZT-1 had a high similarity with that of glycosyl hydrolase family 11 (GHF11) reported from other microorganisms. The DNA sequence encoding mature peptide was subcloned into pET-28a(+) expression vector. The resulted plasmid pET-28axynZT-1 was transformed into E. coli BL21(DE3), and the recombinant strain BL21(DE3)/xynZT-1 was obtained. The optimum temperature and pH of the recombinant XynZT-1 were 45 celcius and 5.0, respectively.

引用

页码：134 / 142

页数：9

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