In vivo 13C spectroscopy in the rat brain using hyperpolarized [1-13C]pyruvate and [2-13C]pyruvate

被引:72
|
作者
Marjanska, Malgorzata [1 ]
Iltis, Isabelle
Shestov, Alexander A.
Deelchand, Dinesh K.
Nelson, Christopher
Ugurbil, Karnil
Henry, Pierre-Gilles
机构
[1] Univ Minnesota, Ctr Magnet Resonance Res, Minneapolis, MN 55455 USA
关键词
DNP; Time courses; Pyruvate; Lactate; Bicarbonate; PARAHYDROGEN-INDUCED POLARIZATION; DYNAMIC NUCLEAR-POLARIZATION; MAGNETIC-RESONANCE; PERFUSION ASSESSMENT; CARDIAC METABOLISM; NMR DETECTION; PYRUVATE; LACTATE; ANGIOGRAPHY; OXIDATION;
D O I
10.1016/j.jmr.2010.07.006
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The low sensitivity of C-13 spectroscopy can be enhanced using dynamic nuclear polarization. Detection of hyperpolarized [1-C-13]pyruvate and its metabolic products has been reported in kidney, liver, and muscle. In this work, the feasibility of measuring C-13 signals of hyperpolarized C-13 metabolic products in the rat brain in vivo following the injection of hyperpolarized [1-C-13]pyruvate and [2-C-13]pyruvate is investigated. Injection of [2-C-13]pyruvate led to the detection of [2-C-13]lactate, but no other downstream metabolites such as TCA cycle intermediates were detected. Injection of [1-C-13]pyruvate enabled the detection of both [1-C-13]lactate and [C-13]bicarbonate. A metabolic model was used to fit the hyperpolarized C-13 time courses obtained during infusion of [1-C-13]pyruvate and to determine the values of V-PDH and V-LDH. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:210 / 218
页数:9
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