Pegylation effect of chitosan based polyplex on DNA transfection

被引：28
作者：
Lin, Wen Jen [1 ,2 ]
Hsu, Wan Yi [1 ]
机构：
[1] Natl Taiwan Univ, Sch Pharm, Grad Inst Pharmaceut Sci, Taipei 100, Taiwan
[2] Natl Taiwan Univ, Coll Med, Drug Res Ctr, Taipei 100, Taiwan
来源：
CARBOHYDRATE POLYMERS | 2015年 / 120卷
关键词：
Chitosan; Galactose; Methoxy poly(ethylene glycol); Poly(ethylene glycol) diacid; DNA transfection; CHEMICAL-MODIFICATION; GENE CARRIER; NANOPARTICLES; BIODISTRIBUTION; CYTOTOXICITY; COPOLYMERS; DELIVERY; RELEASE; GLYCOL); DESIGN;
D O I：
10.1016/j.carbpol.2014.11.046
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The aim of this study was to develop hepatocyte-targeting non-viral polymeric nono-carriers for gene delivery. Chitosan was selected as the main polymer. An asialoglycoprotein receptor recognized sugar, galactose, was introduced. The methoxy poly(ethylene glycol) (mPEG) or short chain poly(ethylene glycol) diacid (PEGd) was further grafted onto galactosylated chitosan. All polyplex possessed positive charge character. The compaction of DNA by grafted chitosan was in order of chitosan-galactose-mPEG > chitosan-galactose-PEGd > chitosan-galactose where the chitosan-galactosemPEG and pDNA formed the most stable polyplex. The polyplex prominently enhanced DNA cellular transfection as compared to naked DNA in HepG2 cells in order of chitosan-galactose/pDNA (11.6 +/- 0.6-33.0 +/- 4.4%) > chitosan-galactose-PEGd/pDNA (12.7 +/- 2.5-15.5 +/- 3.0%) > chitosan-galactosemPEG/p DNA (9.0 +/- 1.1-12.9 +/- 2.4%). (C) 2014 The Authors. Published by Elsevier Ltd.
引用
收藏
页码：7 / 14
页数：8
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