Rapid biosynthesized AgNPs from Gelidiella acerosa aqueous extract mitigates quorum sensing mediated biofilm formation of Vibrio species-an in vitro and in vivo approach

被引:30
作者
Satish, Lakkakula [1 ,2 ]
Santhakumari, Sivasubramanian [1 ]
Gowrishankar, Shanmugaraj [1 ]
Pandian, Shunmugiah Karutha [1 ]
Ravi, Arumugam Veera [1 ]
Ramesh, Manikandan [1 ]
机构
[1] Alagappa Univ, Dept Biotechnol, Sci Campus, Karaikkudi 630003, Tamil Nadu, India
[2] Ben Gurion Univ Nagev, Dept Biotechnol Engn, IL-84105 Beer Sheva, Israel
关键词
Aquaculture; Artemia franciscana; Motility; Quorum sensing; Silver nanoparticles; Violacein; SILVER NANOPARTICLES; ANTIBACTERIAL ACTIVITY; ANTIBIOTIC-RESISTANCE; VIRULENCE FACTORS; PARAHAEMOLYTICUS; INHIBITION; VULNIFICUS; EFFICACY; CYCLO(L-LEUCYL-L-PROLYL); PATHOGENS;
D O I
10.1007/s11356-017-0296-4
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The present study explores the non-bactericidal anti-virulence efficacy of green synthesized silver nanoparticles (AgNPs) from Gelidiella acerosa against multi-drug resistant Vibrio spp. Spectral characterization of AgNPs was performed through UV-Visible, FT-IR, and energy-dispersive spectroscopic techniques followed by X-ray crystallography and zeta potential analysis. Further, the structural characterization was done by electron and atomic force microscopic techniques. AgNPs profoundly quelled the quorum sensing mediated violacein production in Chromobacterium violaceum and CV026. Characterized AgNPs at 100 mu g mL(-1) concentrations depicted a phenomenal anti-biofilm efficacy against Vibrio parahaemolyticus (71%) and Vibrio vulnificus (83%) biofilms, which was further confirmed through light, confocal, and scanning electron microscopic analyses. In vitro bioassays revealed the remarkable inhibitory values of AgNPs, by inhibiting the exopolysaccharide production, hydrophobicity, and motility. In vivo studies using Artemia franciscana larvae also confirmed the anti-infective proficiency, as the AgNPs effectively reduced the bacterial colonization and enhanced the survival rate of larvae up to 100% without any toxicity effect.
引用
收藏
页码:27254 / 27268
页数:15
相关论文
共 58 条
  • [1] [Anonymous], ALTERN MED
  • [2] Synthesis of cerium oxide nanoparticles using Gloriosa superba L. leaf extract and their structural, optical and antibacterial properties
    Arumugam, Ayyakannu
    Karthikeyan, Chandrasekaran
    Hameed, Abdulrahman Syedahamed Haja
    Gopinath, Kasi
    Gowri, Shanmugam
    Karthika, Viswanathan
    [J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2015, 49 : 408 - 415
  • [3] Bhimba BV, 2010, ASIAN PAC J TROP MED, V3, P544
  • [4] Bross MH, 2007, AM FAM PHYSICIAN, V76, P539
  • [5] Biorecovery of gold using cyanobacteria and an eukaryotic alga with special reference to nanogold formation - a novel phenomenon
    Chakraborty, Nabanita
    Banerjee, Anupam
    Lahiri, Susanta
    Panda, Arpita
    Ghosh, Amar Nath
    Pal, Ruma
    [J]. JOURNAL OF APPLIED PHYCOLOGY, 2009, 21 (01) : 145 - 152
  • [6] Interactions of nanosilver with Escherichia coli cells in planktonic and biofilm cultures
    Choi, Okkyoung
    Yu, Chang-Ping
    Fernandez, G. Esteban
    Hu, Zhiqiang
    [J]. WATER RESEARCH, 2010, 44 (20) : 6095 - 6103
  • [7] Inhibition of bacterial quorum sensing by vanilla extract
    Choo, J. H.
    Rukayadi, Y.
    Hwang, J. -K.
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 2006, 42 (06) : 637 - 641
  • [8] Relationship between Expression of the Family of M Proteins and Lipoteichoic Acid to Hydrophobicity and Biofilm Formation in Streptococcus pyogenes
    Courtney, Harry S.
    Ofek, Itzhak
    Penfound, Thomas
    Nizet, Victor
    Pence, Morgan A.
    Kreikemeyer, Bernd
    Podbielbski, Andreas
    Hasty, David L.
    Dale, James B.
    [J]. PLOS ONE, 2009, 4 (01):
  • [9] A Quorum Sensing-Disrupting Brominated Thiophenone with a Promising Therapeutic Potential to Treat Luminescent Vibriosis
    Defoirdt, Tom
    Benneche, Tore
    Brackman, Gilles
    Coenye, Tom
    Sorgeloos, Patrick
    Scheie, Anne Aamdal
    [J]. PLOS ONE, 2012, 7 (07):
  • [10] Devi J. S., 2012, Open Access Scientific Reports, V1, P4, DOI [10.4172/scientificreports.242, DOI 10.4172/SCIENTIFICREPORTS.242]