Applicability of multisyringe chromatography coupled to on-line solid-phase extraction to the simultaneous determination of dicamba, 2,4-D, and atrazine

被引:14
作者
Chavez-Moreno, C. A. [1 ]
Guzman-Mar, J. L. [1 ]
Hinojosa-Reyes, L. [1 ]
Hernandez-Ramirez, A. [1 ]
Ferrer, L. [2 ]
Cerda, V. [2 ]
机构
[1] UANL, Fac Ciencias Quim, San Nicolas De Los Garza 66451, Nuevo Leon, Mexico
[2] Univ Balearic Isl, Dept Chem, Palma De Mallorca 07122, Spain
关键词
Herbicides; Multisyringe chromatography; On-line solid-phase extraction; Monolithic column; Environmental analysis; FLOW-INJECTION; MONOLITHIC COLUMN; PESTICIDES; HERBICIDES; TRIAZINES;
D O I
10.1007/s00216-012-6055-y
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Simultaneous determination of three herbicides (dicamba, 2,4-D, and atrazine) has been achieved by on-line solid-phase extraction (SPE) coupled to multisyringe chromatography (MSC) with UV detection. The preconcentration conditions were optimized; a preconcentration flow rate of 0.5 mL min(-1) and elution at 0.8 mL min(-1) were the optimum conditions. A C-18 (8 mm i.d.) membrane extraction disk conditioned with 0.3 mol L-1 HCl in 0.5 % MeOH was used. A 3-mL sample was preconcentrated, then eluted with 0.43 mL 40:60 water-MeOH. A C-18 monolithic column (25 mm x 4.6 mm) was used for chromatographic separation. Separation of the three compounds was achieved in 10 min by use of 0.01 % aqueous acetic acid-MeOH (60:40) as mobile phase at a flow rate of 0.8 mL min(-1). The limits of detection (LOD) were 13, 57, and 22 mu g L-1 for dicamba, 2,4-D, and atrazine, respectively. The sampling frequency was three analyses per hour, and each analysis consumed only 7.3 mL solvent. The method was applied to spiked water samples, and recovery between 85 and 112 % was obtained. Recovery was significantly better than in the conventional HPLC-UV method. These results indicated the reliability and accuracy of this flow-based method. This is the first time this family of herbicides has been simultaneously analyzed by on-line SPE-MSC using a monolithic column.
引用
收藏
页码:2705 / 2714
页数:10
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