Crystal structure of the PepSY-containing domain of the YpeB protein involved in germination of bacillus spores

被引：8

作者：

Uestok, Fatma Isik ^{[1
]}

Chirgadze, Dimitri Y. ^{[2
]}

Christie, Graham ^{[1
]}

机构：

[1] Univ Cambridge, Inst Biotechnol, Dept Chem Engn & Biotechnol, Cambridge, England

[2] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England

来源：

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS | 2015年 / 83卷 / 10期

关键词：

cortex peptidoglycan; cortex lytic enzyme; SleB; CwlJ; SleL; inhibitory protein; CORTEX PEPTIDOGLYCAN; CATALYTIC DOMAIN; ANTHRACIS; SLEB;

D O I：

10.1002/prot.24868

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The crystal structure of the C-terminal domain of the Bacillus megaterium YpeB protein has been solved by X-ray crystallography to 1.80-angstrom resolution. The full-length protein is essential in stabilising the SleB cortex lytic enzyme in Bacillus spores, and may have a role in regulating SleB activity during spore germination. The YpeB-C crystal structure comprises three tandemly repeated PepSY domains, which are aligned to form an extended laterally compressed molecule. A predominantly positively charged region located in the second PepSY domain may provide a site for protein interactions that are important in stabilising SleB and YpeB within the spore. Proteins 2015; 83:1914-1921. (c) 2015 Wiley Periodicals, Inc.

引用

页码：1914 / 1921

页数：8

共 16 条

[1] PHENIX: a comprehensive Python']Python-based system for macromolecular structure solution [J].

Adams, Paul D. ;

Afonine, Pavel V. ;

Bunkoczi, Gabor ;

Chen, Vincent B. ;

Davis, Ian W. ;

Echols, Nathaniel ;

Headd, Jeffrey J. ;

Hung, Li-Wei ;

Kapral, Gary J. ;

Grosse-Kunstleve, Ralf W. ;

McCoy, Airlie J. ;

Moriarty, Nigel W. ;

Oeffner, Robert ;

Read, Randy J. ;

Richardson, David C. ;

Richardson, Jane S. ;

Terwilliger, Thomas C. ;

Zwart, Peter H. .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2010, 66 :213-221

[2] Electrostatics of nanosystems: Application to microtubules and the ribosome [J].

Baker, NA ;

Sept, D ;

Joseph, S ;

Holst, MJ ;

McCammon, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (18) :10037-10041

[3] HtrC Is Involved in Proteolysis of YpeB during Germination of Bacillus anthracis and Bacillus subtilis Spores [J].

Bernhards, Casey B. ;

Chen, Yan ;

Toutkoushian, Hannah ;

Popham, David L. .

JOURNAL OF BACTERIOLOGY, 2015, 197 (02) :326-336

[4] Role of YpeB in Cortex Hydrolysis during Germination of Bacillus anthracis Spores [J].

Bernhards, Casey B. ;

Popham, David L. .

JOURNAL OF BACTERIOLOGY, 2014, 196 (19) :3399-3409

[5] Analysis of spore cortex lytic enzymes and related proteins in Bacillus subtilis endospore germination [J].

Chirakkal, H ;

O'Rourke, M ;

Atrih, A ;

Foster, SJ ;

Moir, A .

MICROBIOLOGY-SGM, 2002, 148 :2383-2392

[6] The structure of BVU2987 from Bacteroides vulgatus reveals a superfamily of bacterial periplasmic proteins with possible inhibitory function [J].

Das, Debanu ;

Finn, Robert D. ;

Carlton, Dennis ;

Miller, Mitchell D. ;

Abdubek, Polat ;

Astakhova, Tamara ;

Axelrod, Herbert L. ;

Bakolitsa, Constantina ;

Chen, Connie ;

Chiu, Hsiu-Ju ;

Chiu, Michelle ;

Clayton, Thomas ;

Deller, Marc C. ;

Duan, Lian ;

Ellrott, Kyle ;

Ernst, Dustin ;

Farr, Carol L. ;

Feuerhelm, Julie ;

Grant, Joanna C. ;

Grzechnik, Anna ;

Han, Gye Won ;

Jaroszewski, Lukasz ;

Jin, Kevin K. ;

Klock, Heath E. ;

Knuth, Mark W. ;

Kozbial, Piotr ;

Krishna, S. Sri ;

Kumar, Abhinav ;

Marciano, David ;

McMullan, Daniel ;

Morse, Andrew T. ;

Nigoghossian, Edward ;

Nopakun, Amanda ;

Okach, Linda ;

Puckett, Christina ;

Reyes, Ron ;

Rife, Christopher L. ;

Sefcovic, Natasha ;

Tien, Henry J. ;

Trame, Christine B. ;

van den Bedem, Henry ;

Weekes, Dana ;

Wooten, Tiffany ;

Xu, Qingping ;

Hodgson, Keith O. ;

Wooley, John ;

Elsliger, Marc-Andre ;

Deacon, Ashley M. ;

Godzik, Adam ;

Lesley, Scott A. .

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2010, 66 :1265-1273

[7] MolProbity: structure validation and all-atom contact analysis for nucleic acids and their complexes [J].

Davis, IW ;

Murray, LW ;

Richardson, JS ;

Richardson, DC .

NUCLEIC ACIDS RESEARCH, 2004, 32 :W615-W619

[8] Searching protein structure databases with DaliLite v.3 [J].

Holm, L. ;

Kaariainen, S. ;

Rosenstrom, P. ;

Schenkel, A. .

BIOINFORMATICS, 2008, 24 (23) :2780-2781

[9] The catalytic domain of the germination-specific lytic transglycosylase SleB from Bacillus anthracis displays a unique active site topology [J].

Jing, Xing ;

Robinson, Howard R. ;

Heffron, Jared D. ;

Popham, David L. ;

Schubot, Florian D. .

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2012, 80 (10) :2469-2475

[10] Activity and Regulation of Various Forms of CwlJ, SleB, and YpeB Proteins in Degrading Cortex Peptidoglycan of Spores of Bacillus Species In Vitro and during Spore Germination [J].

Li, Yunfeng ;

Butzin, Xuan Y. ;

Davis, Andrew ;

Setlow, Barbara ;

Korza, George ;

Uestok, Fatma Isik ;

Christie, Graham ;

Setlow, Peter ;

Hao, Bing .

JOURNAL OF BACTERIOLOGY, 2013, 195 (11) :2530-2540

← 1 2 →