Stabilities of cubane type [Fe4S4(SR)4]2- clusters in partially aqueous media

被引:12
作者
Lo, Wayne [1 ]
Scott, Thomas A. [1 ]
Zhang, Ping [2 ]
Ling, Chang-Chun [2 ]
Holm, R. H. [1 ]
机构
[1] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
[2] Univ Calgary, Dept Chem, Alberta Ingenu Ctr Carbohydrate Sci, Calgary, AB T2N 1N4, Canada
关键词
Iron-sulfur clusters; Cyclodextrin thiolates; Solution stability of clusters; IRON-SULFUR PROTEINS; SEQUENCE-SPECIFIC ASSIGNMENT; MAGNETIC CIRCULAR-DICHROISM; ACTIVE-SITES; SYNTHETIC ANALOGS; CRYSTAL-STRUCTURE; 4FE-4S CLUSTER; LIGAND; FERREDOXIN; CORE;
D O I
10.1016/j.jinorgbio.2010.12.009
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The stability of cubane-type [Fe4S4(SR)(4)](2-) clusters in mixed organic/aqueous solvents was examined as an initial step in the development of stable water-soluble cluster compounds possibly suitable for reconstitution of scaffold proteins in protein biosynthesis. The research involves primarily spectrophotometric assessment of stability in 20-80% Me2SO/aqueous media (v/v), from which it was found that conventional clusters tend to be stable for up to 12 h in 60% Me2SO but are much less stable at higher aqueous content. alpha-Cyclodextrin mono- and dithioesters and thiols were prepared as ligand precursors for cluster binding, which was demonstrated by spectroscopic methods. A potentially bidentate cyclodextrin dithiolate was found to be relatively effective for cluster stabilization in 40% Me2SO, suggesting (together with earlier results) that other exceptionally large thiolate ligands may promote cluster stability in aqueous media. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:497 / 508
页数:12
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