Location analysis of DNA-bound proteins at the whole-genome level: untangling transcriptional regulatory networks

被引:17
|
作者
Nal, B [1 ]
Mohr, E [1 ]
Ferrier, P [1 ]
机构
[1] Univ Medietteranee, CIML, CNRS, INSERM, F-13288 Marseille 9, France
关键词
D O I
10.1002/bies.1066
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this post-sequencing era, geneticists can focus on functional genomics an a much larger scale than ever before. One goal is the discovery and elucidation of the intricate genetic networks that co-ordinate transcriptional activation in different regulatory circuitries. High-throughput gene expression measurement using DNA arrays has thus become routine strategy. This approach, however, does not directly identify gene loci that belong to the same regulatory group; e,g,, those that are bound by a common (set of) transcription factor(s), Working in yeast, two groups have recently published an elegant method that could circumvent this problem, by combining chromatin immunoprecipitation and DNA micro-arrays.((1,2)) The method is likely to provide a powerful arrays. tool for the dissection of global regulatory networks in eukaryotic cells. (C) 2001 John Wiley & Sons, Inc.
引用
收藏
页码:473 / 476
页数:4
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