Expression and purification of recombinant G protein-coupled receptors: A review

被引：44

作者：

Wiseman, Daniel N. ^{[1
]}

Otchere, Abigail ^{[1
]}

Patel, Jaimin H. ^{[1
]}

Uddin, Romez ^{[1
]}

Pollock, Naomi L. ^{[2
]}

Routledge, Sarah J. ^{[1
]}

Rothnie, Alice J. ^{[1
]}

Slack, Cathy ^{[1
]}

Poyner, David R. ^{[1
]}

Bill, Roslyn M. ^{[1
]}

Goddard, Alan D. ^{[1
]}

机构：

[1] Aston Univ, Sch Life & Hlth Sci, Birmingham B4 7ET, W Midlands, England

[2] Univ Birmingham, Birmingham B15 2TT, W Midlands, England

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2020年 / 167卷

基金：

英国生物技术与生命科学研究理事会; 欧盟地平线“2020”;

关键词：

Expression; Purification; GPCR; SMALP; Review; PERIPHERAL CANNABINOID RECEPTOR; METABOTROPIC GLUTAMATE-RECEPTOR; DETERGENT-FREE ISOLATION; N-LINKED GLYCOSYLATION; MEMBRANE-PROTEINS; ESCHERICHIA-COLI; PICHIA-PASTORIS; LIGAND-BINDING; PHARMACOLOGICAL CHARACTERIZATION; SCHIZOSACCHAROMYCES-POMBE;

D O I：

10.1016/j.pep.2019.105524

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this, many of these receptors have limited or no available prophylaxis. Novel drug design and discovery significantly rely on structure determination, of which GPCRs are typically elusive. Progress has been made thus far to produce sufficient quantity and quality of protein for downstream analysis. As such, this review highlights the systems available for recombinant GPCR expression, with consideration of their advantages and disadvantages, as well as examples of receptors successfully expressed in these systems. Additionally, an overview is given on the use of detergents and the styrene maleic acid (SMA) co-polymer for membrane solubilisation, as well as purification techniques.

引用

页数：10

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