CEA gene element control suicide gene therapy mediated by the adenovirus vector.

被引：0

作者：

Xu, DH ^{[1
]}

Ge, K ^{[1
]}

Jiang, Q ^{[1
]}

Zheng, ZC ^{[1
]}

Liu, XY ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 1998年 / 25卷 / 05期

关键词：

adenovirus vector; CEA promoter; herpes simplex virus thymidine kinase (HSV-TK) gene; gene therapy;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A replication-defective recombinant adenovirus vector containing HSV-TK gene under the control of CEA promoter was constructed (AdCEATK). Titer of the purified recombinant adenovirus is about 10(12) pfu/ml, HeLa cell (CEA-negative cell) infected with AdCMVTK became sensitive to GCV, while HeLa cell infected with AdCEATK was not. On the contrary, the LoVo cell infected with both AdCMVTK and AdCEATK were sensitive to GCV. It was shown that CEA promoter has a good tumor-specificity. Significant bystander effect was observed in the AdCEATK/GCV system too. This system should be useful for tumor-specificity suicide gene therapy of CEA-positive tumors.

引用

页码：444 / 448

页数：5