Novel bioconversion products of andrographolide by Aspergillus ochraceus and their cytotoxic activities against human tumor cell lines

被引：9

作者：

He, Xiangjiu ^{[1
]}

Wang, Yihai ^{[1
]}

Hu, Hui ^{[1
]}

Wu, Yixuan ^{[1
]}

Zeng, Xiaobin ^{[1
]}

机构：

[1] Wuhan Univ, Coll Pharm, Wuhan 430071, Peoples R China

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2011年 / 68卷 / 01期

关键词：

Andrographolide; Aspergillus ochraceus; Hydroxylation; Microbial conversion; Cytotoxicity; CYCLE ARREST; APOPTOSIS; INTERFERES; PROTECTS; PHASE;

D O I：

10.1016/j.molcatb.2010.09.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Andrographolide (1) a major labdane diterpenoidal constituent of a famous traditional Chinese of Andrographis paniculata exhibits a wide spectrum of biological activities including antibacterial, anti-inflammatory and antitumor properties Bioconversion of andrographolide (1) by Aspergillus ochraceus (ATCC 1008) was investigated Five bioconversion products were isolated and identified Their structures were identified to be 8 beta-hydroxy-8(17)-dihydroandrographolide (2), 8 beta-hydroxy-8(17)-dihydro-14-deoxy-11 12-didehydroandrographohde (3) 80-hydroxy-8(17)-dihydro-14-deoxy-11 12-didehydroandrographolide 19-oic acid (4). 14-deoxy-11 12-didehydroandrographolide (5), and 14-deoxy-11 12-didehydroandrographolide 19-oic acid (6) Metabolites 2-4 were novel compounds The proposed biosynthetic pathways of andrographolide by A ochraceus were drawn Most bloconversion products showed potential cytotoxic activities against human breast cancer (MCF-7) human colon cancer (HCT-116) and leukemia (HL-60) cell lines (c) 2010 Elsevier B V All rights reserved

引用

页码：89 / 93

页数：5

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