Use of a BJAB-derived cell line for isolation of human herpesvirus 8

被引:17
作者
Gasperini, P
Barbierato, M
Martinelli, C
Rigotti, P
Marchini, F
Masserizzi, G
Leoncini, F
Chieco-Bianchi, L
Schulz, TF
Calabrò, ML
机构
[1] Univ Padua, Dept Oncol & Surg Sci, Oncol Sect, I-35128 Padua, Italy
[2] Gen Hosp Careggi, Infect Dis Unit, Florence, Italy
[3] Univ Padua, Dept Med & Surg Sci, I-35128 Padua, Italy
[4] Azienda Osped, Div Nephrol 2, Padua, Italy
[5] Hannover Med Sch, Dept Virol, D-3000 Hannover, Germany
关键词
D O I
10.1128/JCM.43.6.2866-2875.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Establishment of latently infected cell lines from primary effusion lymphomas (PEL) presently is the most efficient system for the propagation of clinical strains of human herpesvirus 8 (HHV-8) in culture. Here we describe a new approach to culture productively replicating HHV-8 from patient samples. A BJAB-derived B-cell line, BBF, was found to retain HHV-8 longer, to support the latent and lytic replication programs, and to produce transmissible virus. Supernatants from n-butyrate-treated peripheral blood mononuclear cells of 24 HHV-8-seropositive renal transplant recipients were used to infect BBF cells, and replicating virus was detected in cultures from 11 patients. Moreover, BBF cells infected with saliva strains showed a highly productive profile regardless of the initial viral load, which confirms that infectious HHV-8 can be present in saliva and also suggests that saliva strains may exhibit a high tropism for B lymphocytes. In conclusion, we established an in vitro system that efficiently detects HRV-8 in samples with low viral loads and that produces infectious progeny. BBF cells can be used to propagate HHV-8 from different biological samples as well as to clarify important issues related to virus-cell interactions in a context distinct from endothelial and PEL-derived cell lines.
引用
收藏
页码:2866 / 2875
页数:10
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