Monoammonium glycyrrhizinate inhibited the inflammation of LPS-induced acute lung injury in mice

Monoammonium glycyrrhizinate (MAC) was the aglycone of glycyrrhizin derived from licorice. In this study, the anti-inflammatory effects of MAC on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice and the possible mechanisms involved in this protection were investigated. Pretreatment with MAC prior to the administration of intratracheal LPS significantly induced a decrease in lung wet weight/dry weight ratio, in total leukocyte number and neutrophil percent in the BALF, and in myeloperoxidase (MPO) activity of lung in dose-dependent manners. At the same time, pretreatment with MAC also significantly improved the super oxide dismutase (SOD) activity and induced the malondialdehyde (MDA) content in the bronchoalveolar lavage fluid (BALF). Importantly, pretreatment with MAC prevented an increase in cyclic adenosine monophosphate-phosphodiesterase (cAMP-PDE) activity of lung in a dose-dependent manner. In addition, it can up-regulate the interleukin-10 (IL-10) level and down-regulate the tumor neurosis factor-alpha (TNF-alpha) level in the lung tissue of ALI mice. These results showed that anti-inflammatory effects of MAC against the LPS-induced ALI may be due to its ability of primary inhibition of cAMP-PDE activity, oxidative stress and its regulation of cytokine effects. Thus the results support that use of MAC is beneficial in the treatment of ALI and ARDS. (C) 2010 Elsevier B.V. All rights reserved.