De novo biosynthesis of alpha-zingiberene from glucose in Escherichia coli

被引：2

作者：

Zhang, Suping ^{[1
]}

Zhao, Xin ^{[1
]}

Hu, Die ^{[1
]}

Qi, Yonggang ^{[1
]}

Zhou, Mengzhou ^{[1
]}

Li, Dongsheng ^{[1
]}

Hua, Qiang ^{[2
]}

Wu, Yongchao ^{[3
]}

Liu, Zhijie ^{[1
]}

机构：

[1] Hubei Univ Technol, Hubei Collaborat Innovat Ctr Ind Fermentat, Key Lab Fermentat Engn, Hubei Key Lab Ind Microbiol,Minist Educ, Wuhan 430068, Peoples R China

[2] East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

[3] Hubei Juhui Agr Co Ltd, Jingmen 431821, Peoples R China

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2021年 / 176卷

基金：

中国国家自然科学基金;

关键词：

Escherichia coli; Metabolic engineering; Alpha-zingiberene; MVA pathway; ESSENTIAL OIL; IN-VITRO; PATHWAY; LYCOPENE; GROWTH; MVA;

D O I：

10.1016/j.bej.2021.108188

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Alpha-zingiberene, a functionally important active component of ginger, is used extensively in various applications in the fields of medicine, food, and agriculture. In this study, alpha-zingiberene was de novo bio-synthesized from glucose using metabolically engineered Escherichia coli as hosts. First, the alpha-zingiberene synthase, VvPNaZin, was heterologously expressed in an E. coli strain. The resultant recombinant strain, LZ020, produced 0.012 +/- 0.002 mg/L of alpha-zingiberene. Next, ERG20 was overexpressed in LZ021 to increase the farnesyl pyrophosphate (FPP) content, which enhanced the yield of alpha-zingiberene to 0.041 +/- 0.017 mg/L. Finally, the heterologous mevalonate (MVA) pathway was introduced to increase the supply of dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP) precursors, which induced LZ022 to produce 0.764 +/- 0.048 mg/L of alpha-zingiberene, representing a 63.7-fold increase compared to that produced by the LZ020 strain. Thus, the current study established a technique that facilitates microbial synthesis of alpha-zingiberene directly from glucose, thereby providing methodology which enables sustainable production of alpha-zingiberene.

引用

页数：5

共 25 条

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