Testing mitochondrial marker efficacy for DNA barcoding in spiders: a test case using the dwarf spider genus Oedothorax (Araneae : Linyphiidae : Erigoninae)

被引:12
|
作者
Lopardo, Lara [1 ]
Uhl, Gabriele [1 ]
机构
[1] Univ Greifswald, Zool Inst & Museum, Anklamer Str 20, D-17489 Greifswald, Germany
关键词
biodiversity; species boundaries; mitochondrial DNA; molecular taxonomy; C-OXIDASE SUBUNIT-1; SPECIES DELIMITATION; IDENTIFYING SPIDERS; MOLECULAR PHYLOGENETICS; GENETIC-DIVERGENCE; GIBBOSUS BLACKWALL; TRAPDOOR SPIDERS; MALE DIMORPHISM; TAXONOMY; MYGALOMORPHAE;
D O I
10.1071/IS14017
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The present study focusses on comparatively assessing the efficacy for DNA barcoding of the two most commonly used mitochondrial markers (cox1 and 16S) in a genus of erigonine spiders. In total, 53 specimens representing five species, including four multi-sampled species, were sampled from several European localities. Initial evaluation of species monophyly was performed through parsimony and Bayesian phylogenetic analyses. Efficacy of mitochondrial markers was tested using operational (including distance-, tree-based measures and Barcode Gap) and evolutionary criteria (using the General Mixed Yule-coalescent Model) for species delimitation. We propose that the cox1 marker can potentially overestimate analyses of biodiversity and thus might not be the preferred marker for DNA species identification and delimitation methods in Oedothorax. Instead, our results suggest that the 16S marker appears to be a promising candidate for such endeavour. Evaluating the contribution and suitability of markers to the re-identification of species, measured by their recovery of well established morphological species, is critical for future studies and for reliable results in species identification in spiders.
引用
收藏
页码:501 / 521
页数:21
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