Development of In Situ Hybridization for the Detection of Mycoplasma hyorhinis in Formalin-Fixed Paraffin-Embedded Tissues from Naturally Infected Pigs with Polyserositis

被引：6

作者：

Kim, Bongtae ^{[1
]}

Lee, Kichan ^{[1
]}

Han, Kiwon ^{[1
]}

Kim, Duyeol ^{[1
]}

Ha, Yooncheol ^{[1
]}

Kim, Chung Hyun ^{[1
]}

Oh, Yeonsu ^{[1
]}

Kang, Ikjae ^{[2
]}

Lee, Jeehoon ^{[2
]}

Chae, Chanhee ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Vet Pathol, Coll Vet Med, Seoul 151742, South Korea

[2] Dong Bang Co Ltd, Seoul 135272, South Korea

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2010年 / 72卷 / 09期

关键词：

in situ hybridization; Mycoplasma hyorhinis; swine; LINKED IMMUNOSORBENT-ASSAY; INDIRECT HEMAGGLUTINATION; COMPLEMENT-FIXATION; HYOPNEUMONIAE; DIFFERENTIATION; HYOSYNOVIAE; TESTS; SWINE;

D O I：

10.1292/jvms.10-0062

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The aim of this study was to develop in situ hybridization for detection of Mycoplasma hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from pigs with polyserositis. M hyorhinis was isolated from the spleen (2 pigs) and pericardium (1 pig). M. hyorhinis DNA was detected 16 out of 20 pigs with polyserositis. In situ hybridization produced a distinct positive signal for the M. hyorhinis p37 gene in inflammatory cells in the polyserositis. In situ hybridization developed in the present study present diagnostic tools capable of detection of M. hyorhinis in formalin-fixed, paraffin-wax-embedded tissues from the naturally infected pigs.

引用

页码：1225 / 1227

页数：3

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