Duplex PCR to differentiate between Mycoplasma synoviae and Mycoplasma gallisepticum on the basis of conserved species-specific sequences of their hemagglutinin genes

被引：28

作者：

Mardassi, BBA ^{[1
]}

Ben Mohamed, R ^{[1
]}

Gueriri, I ^{[1
]}

Boughattas, S ^{[1
]}

Mlik, B ^{[1
]}

机构：

[1] Inst Pasteur, Lab Mycoplasmes, Tunis 1002, Tunisia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 02期

关键词：

D O I：

10.1128/JCM.43.2.948-950.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a duplex PCR assay targeting the hemagglutinin multigene families, vlhA and pMGA, of Mycoplasma synoviae and Mycoplasma gallisepticum, respectively. The assay proved to be specific and sensitive enough to justify its use for the simultaneous detection of the two major avian mycoplasma species from field isolates.

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页码：948 / 950

页数：3

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