1H, 13C, 15N backbone NMR assignments of the Staphylococcus aureus small multidrug-resistance pump (Smr) in a functionally active conformation

被引：12
作者：
Poget, Sebastien F. [2 ]
Harris, Richard [1 ]
Cahill, Sean M. [1 ]
Girvin, Mark E. [1 ]
机构：
[1] Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10461 USA
[2] CUNY Coll Staten Isl, Dept Chem, Staten Isl, NY 10314 USA
来源：
BIOMOLECULAR NMR ASSIGNMENTS | 2010年 / 4卷 / 02期
关键词：
MDR; Smr; NMR; Bicelle; Membrane protein; TRANSPORTER EMRE; MEMBRANE-PROTEIN; MODEL; EVOLUTION; TOPOLOGY;
D O I：
10.1007/s12104-010-9228-7
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The plasmid-encoded small multidrug resistance pump from S. aureus transports a variety of quaternary ammonium and other hydrophobic compounds, enhancing the bacterial host's resistance to common hospital disinfectants. The protein folds as a homo-dimer of four transmembrane helices each, and appears to be fully functional only in lipid bilayers. Here we report the backbone resonance assignments and implied secondary structure for (HCN)-H-2-C-13-N-15 Smr reconstituted into lipid bicelles. Significant changes were observed between the chemical shifts of the protein in lipid bicelles compared to those in detergent micelles.
引用
收藏
页码：139 / 142
页数：4
相关论文
共 50 条
  • [11] 1H, 13C and 15N NMR spectral assignments for new triazapentalene derivatives
    Palmas, Pascal
    Nyffenegger, Coralie
    Pasquinet, Eric
    Guillaumet, Gerald
    MAGNETIC RESONANCE IN CHEMISTRY, 2009, 47 (09) : 752 - 756
  • [12] 1H, 13C, and 15N NMR assignments of a Drosophila Hedgehog autoprocessing domain
    Jian Xie
    Zhenming Du
    Brian Callahan
    Marlene Belfort
    Chunyu Wang
    Biomolecular NMR Assignments, 2014, 8 : 279 - 281
  • [13] 1H, 13C and 15N backbone and side chain resonance assignments of human interleukin 1α
    Chang, Hsuan-Kuo
    Mohan, Sepuru K.
    Chin, Yu
    BIOMOLECULAR NMR ASSIGNMENTS, 2010, 4 (01) : 59 - 60
  • [14] 1H, 15N and 13C backbone resonance assignments of the archetypal serpin α1-antitrypsin
    Nyon, Mun Peak
    Kirkpatrick, John
    Cabrita, Lisa D.
    Christodoulou, John
    Gooptu, Bibek
    BIOMOLECULAR NMR ASSIGNMENTS, 2012, 6 (02) : 153 - 156
  • [15] 1H, 13C and 15N backbone and side chain resonance assignments of human interleukin 1α
    Hsuan-Kuo Chang
    Sepuru K. Mohan
    Yu Chin
    Biomolecular NMR Assignments, 2010, 4 : 59 - 60
  • [16] Letter to the Editor: Backbone and Sidechain 1H, 13C and 15N Resonance Assignments of Human Cofilin
    Karen M. Zierler-Gould
    Brian J. Pope
    Alan G. Weeds
    Linda J. Ball
    Journal of Biomolecular NMR, 2004, 29 : 429 - 430
  • [17] 1H, 13C and 15N backbone resonance assignments and dynamic properties of the PDZ tandem of Whirlin
    Delhommel, Florent
    Wolff, Nicolas
    Cordier, Florence
    BIOMOLECULAR NMR ASSIGNMENTS, 2016, 10 (02) : 361 - 365
  • [18] 1H, 13C, and 15N resonance assignments of human ζ-COP
    Yu, Wenyu
    Jin, Changwen
    Xia, Bin
    BIOMOLECULAR NMR ASSIGNMENTS, 2008, 2 (01) : 73 - 75
  • [19] 1H, 13C, and 15N resonance assignments of human ζ-COP
    Wenyu Yu
    Changwen Jin
    Bin Xia
    Biomolecular NMR Assignments, 2008, 2 : 73 - 75
  • [20] Letter to the Editor:: Backbone and sidechain 1H, 13C and 15N resonance assignments of human cofilin
    Zierler-Gould, KM
    Pope, BJ
    Weeds, AG
    Ball, LJ
    JOURNAL OF BIOMOLECULAR NMR, 2004, 29 (03) : 429 - +
12345