Ultrastructural and metabolic effects after vitrification of precision-cut rat liver slices with antifreeze proteins

被引：0
|
作者：
Ekins, S
Murray, GI
Hawksworth, GM
机构：
[1] UNIV ABERDEEN,DEPT MED & THERAPEUT,ABERDEEN AB9 2ZD,SCOTLAND
[2] UNIV ABERDEEN,DEPT BIOMED SCI,ABERDEEN AB9 2ZD,SCOTLAND
[3] UNIV ABERDEEN,DEPT PATHOL,ABERDEEN AB9 2ZD,SCOTLAND
来源：
CRYO-LETTERS | 1996年 / 17卷 / 03期
关键词：
rat liver slices; vitrification; cryocassette; antifreeze proteins; 7-ethoxycoumarin; electron microscopy;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Antifreeze proteins I and III were utilised in an attempt to prevent loss of cytosolic sulphotransferase activity in vitrified precision-cut rat liver slices after 24hr storage at -196 degrees C. The effect of these novel vitrification regimes, on retention of phase I and II xenobiotic metabolic pathways, were quantified using 7-ethoxycoumarin and 7-hydroxycoumarin as substrates. Hepatic ultrastructure was assessed using transmission electron microscopy before and after vitrification, then compared with the metabolic effects.
引用
收藏
页码：157 / 164
页数：8
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