Ultrastructural and metabolic effects after vitrification of precision-cut rat liver slices with antifreeze proteins

被引：0
|
作者：
Ekins, S
Murray, GI
Hawksworth, GM
机构：
[1] UNIV ABERDEEN,DEPT MED & THERAPEUT,ABERDEEN AB9 2ZD,SCOTLAND
[2] UNIV ABERDEEN,DEPT BIOMED SCI,ABERDEEN AB9 2ZD,SCOTLAND
[3] UNIV ABERDEEN,DEPT PATHOL,ABERDEEN AB9 2ZD,SCOTLAND
来源：
CRYO-LETTERS | 1996年 / 17卷 / 03期
关键词：
rat liver slices; vitrification; cryocassette; antifreeze proteins; 7-ethoxycoumarin; electron microscopy;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Antifreeze proteins I and III were utilised in an attempt to prevent loss of cytosolic sulphotransferase activity in vitrified precision-cut rat liver slices after 24hr storage at -196 degrees C. The effect of these novel vitrification regimes, on retention of phase I and II xenobiotic metabolic pathways, were quantified using 7-ethoxycoumarin and 7-hydroxycoumarin as substrates. Hepatic ultrastructure was assessed using transmission electron microscopy before and after vitrification, then compared with the metabolic effects.
引用
收藏
页码：157 / 164
页数：8
相关论文
共 50 条
  • [1] Vitrification of precision-cut rat liver slices
    Ekins, S
    CRYO-LETTERS, 1996, 17 (01) : 7 - 14
  • [2] Cryopreservation of rat precision-cut liver and kidney slices by rapid freezing and vitrification
    de Graaf, Inge A. M.
    Draaisma, Annelies L.
    Schoeman, Olaf
    Fahy, Gregory M.
    Groothuis, Geny M. M.
    Koster, Henk J.
    CRYOBIOLOGY, 2007, 54 (01) : 1 - 12
  • [3] Ultrastructural changes in hepatocytes of precision-cut rat liver slices after incubation for 24 and 48 hours
    Neupert, D
    Glöckner, R
    Neupert, G
    Müller, D
    EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY, 2003, 54 (5-6) : 481 - 488
  • [4] Effects of herbal remedies on precision-cut rat liver slices.
    Johnson, JM
    Ulreich, JB
    JOURNAL OF INVESTIGATIVE MEDICINE, 2003, 51 : S134 - S134
  • [5] Metabolism of zearalenone in precision-cut rat liver slices
    Damm, G.
    Pfeiffer, E.
    Metzler, M.
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2009, 379 : 63 - 63
  • [6] Xenobiotic metabolism in rat, dog, and human precision-cut liver slices, freshly isolated hepatocytes, and vitrified precision-cut liver slices
    Ekins, S
    Williams, JA
    Murray, GI
    Burke, MD
    Marchant, NC
    Engeset, J
    Hawksworth, GM
    DRUG METABOLISM AND DISPOSITION, 1996, 24 (09) : 990 - 995
  • [7] THE HEPATOTOXICITY OF CHLOROFORM IN PRECISION-CUT RAT-LIVER SLICES
    AZRIMEEHAN, S
    MATA, HP
    GANDOLFI, AJ
    BRENDEL, K
    TOXICOLOGY, 1992, 73 (03) : 239 - 250
  • [8] The toxic mechanism and metabolic effects of atractyloside in precision-cut pig kidney and liver slices
    David K. Obatomi
    Nguyen T. K. Thanh
    Stephen Brant
    Peter H. Bach
    Archives of Toxicology, 1998, 72 : 524 - 530
  • [9] The toxic mechanism and metabolic effects of atractyloside in precision-cut pig kidney and liver slices
    Obatomi, DK
    Thanh, NTK
    Brant, S
    Bach, PH
    ARCHIVES OF TOXICOLOGY, 1998, 72 (08) : 524 - 530
  • [10] In-vitro metabolic studies of tacrolimus using precision-cut rat and human liver slices
    Ueda, S
    Cook, M
    Alak, AM
    JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1996, 15 (03) : 349 - 357
12345