NS deletions convert the 2009-H1N1 pandemic virus into a live attenuated vaccine

被引：0

作者：

Zhou, Bin ^{[1
,2
]}

Li, Yan ^{[1
,3
]}

Belser, Jessica A. ^{[4
]}

Pearce, Melissa B. ^{[4
]}

Schmolke, Mirco ^{[5
]}

Subba, Anju ^{[1
]}

Shi, Zhengli ^{[3
]}

Zaki, Sherif R. ^{[6
]}

Blau, Dianna M. ^{[6
]}

Garcia-Sastre, Adolfo ^{[5
,7
,8
]}

Tumpey, Terrence M. ^{[4
]}

Wentworth, David E. ^{[1
,2
]}

机构：

[1] SUNY Albany, Sch Publ Hlth, New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12222 USA

[2] SUNY Albany, Sch Publ Hlth, Dept Biomed Sci, Albany, NY USA

[3] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Peoples R China

[4] Ctr Dis Control & Prevent, Immunol & Pathogenesis Branch, Influenza Div, NCIRD, Atlanta, GA USA

[5] Mt Sinai Sch Med, Dept Microbiol, New York, NY USA

[6] Ctr Dis Control & Prevent, Infect Dis Pathol Branch, Atlanta, GA USA

[7] Mt Sinai Sch Med, Inst Global Hlth & Emerging Pathogens, New York, NY USA

[8] Mt Sinai Sch Med, Dept Med, Div Infect Dis, New York, NY USA

来源：

INFLUENZA AND OTHER RESPIRATORY VIRUSES | 2011年 / 5卷

关键词：

H1N1; live attenuated; NS; pandemic; vaccine; INFLUENZA-A VIRUS; ADAPTIVE IMMUNITY; PROTEIN; RESPONSES; INNATE; SYSTEM; CELLS;

D O I：

暂无

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Although vaccines against influenza A virus are the most effective method by which to combat infection, it is clear that their production needs to be accelerated and their efficacy improved. A panel of recombinant live attenuated human influenza A vaccines (LAIVs), including NS1-73, NS1-126, NSD5, were generated by rationally engineering mutations directly into the genome of a pandemic-H1N1 virus. The vaccine potential of each LAIV was determined through analysis of attenuation, immunogenicity, and their ability to protect mice and ferrets. The data indicate that the novel NSD5-LAIV was ideally attenuated and elicited strong protective immunity. This study also shows that attenuating mutations can be rapidly engineered into the genomes of emerging/circulating influenza A viruses in order to produce LAIVs.

引用

页码：388 / 391

页数：4

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