Continuous acetone-ethanol-butanol fermentation by immobilized cells of Clostridium acetobutylicum

被引:48
作者
Badr, HR [1 ]
Toledo, R [1 ]
Hamdy, MK [1 ]
机构
[1] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA
关键词
batch and continuous AEB fermentation; immobilized cells of P-262; multistage bioreactor; sweet potato; productivity and yield;
D O I
10.1016/S0961-9534(00)00068-4
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Eight Clostridium acetobutylicum strains were examined for cc-amylase and strains B-591, B-594 and P-262 had the highest activities. Defibered-sweet-potato-slurry (DSPS), containing 39.7 g starch l(-1), supplemented with potassium phosphate (1.0 gl(-1)), cysteine-HCl (5.0 gl(-1)), the antifoam (polypropylene glycol, 0.1 mg ml(-1)), was used as continuous feedstock (FS) to a multistage bioreactor system for acetone-ethanol-butanol (AEB) fermentation. The system consisted of four columns (three vertical and one near horizontal) packed with beads containing immobilized cells of C. acetobutylicum P-262. When DSPS was pumped into the bioreactor system, at a flow rate of 2.36 ml min(-1), the effluent had 7.73 g solvents l(-1) (1.56, acetone; 0.65, ethanol; 5.52 g, butanol) and no starch. Productivity of total solvents synthesized during continuous operation were 1.0 gl(-1) h(-1) and 19.5% yield compared to 0.12 gl(-1) h(-1) with 29% yield using the batch system. We propose using DSPS for AEB fermentation in a continuous mode with immobilized P-262 cells that are active amylase producers which will lead to cost reduction compared to the batch system. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:119 / 132
页数:14
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