Mapping of porcine genes belonging to two different cytochrome P450 subfamilies

被引：28

作者：

Nissen, PH ^{[1
]}

Wintero, AK ^{[1
]}

Fredholm, M ^{[1
]}

机构：

[1] Royal Vet & Agr Univ, Dept Anim Sci & Anim Hlth, Copenhagen, Denmark

来源：

ANIMAL GENETICS | 1998年 / 29卷 / 01期

关键词：

comparative mapping; CYP2C; CYP3A; cytochrome P450; linkage mapping; pig; regional assignment;

D O I：

10.1046/j.1365-2052.1998.00225.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Three cDNA clones from a porcine small intestine cDNA library, tentatively identified as transcripts of two different cytochrome P450 (CYP) gene subfamilies, were sequenced and mapped. One of the clones was found to comprise an open reading frame of 503 amino acids and showed up to an 80% similarity to genes from the CYP3A subfamily. Translation of the other two cDNA clones revealed high similarity to genes from the CYP2C subfamiliy. One of these clones was truncated, lacking a part of the 5' end of the coding region, whereas the other encodes a putative pseudogene. The CYP3A gene was mapped cytogenetically by means of a pig/rodent somatic hybrid cell panel to pig chromosome 3, in the region p16-p17 or p11. The CYP2C pseudogene was mapped genetically to pig chromosome 14, using the PiGMaP shared reference pedigrees. The localisations provide information for the human/pig comparative map.

引用

页码：7 / 11

页数：5

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