Glycine betaine improves oxidative stress tolerance and biocontrol efficacy of the antagonistic yeast Cystofilobasidium infirmominiatum

被引：105
作者：
Liu, Jia [1 ]
Wisniewski, Michael [1 ]
Droby, Samir [2 ]
Vero, Silvana [4 ]
Tian, Shiping [3 ]
Hershkovitz, Vera [2 ]
机构：
[1] USDA ARS, Kearneysville, WV 25430 USA
[2] Agr Res Org, Volcani Ctr, IL-50250 Bet Dagan, Israel
[3] Chinese Acad Sci, Inst Bot, Key Lab Photosynthesis & Environm Mol Physi, Beijing 100093, Peoples R China
[4] Univ Republica, Fac Quim, Inst Catedra Microbiol, Montevideo, Uruguay
来源：
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2011年 / 146卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Glycine betaine; Oxidative stress tolerance; ROS; Antioxidant enzymes; Biocontrol efficacy; METHYLGLYOXAL DETOXIFICATION SYSTEMS; PANTOEA-AGGLOMERANS CPA-2; SWEET CHERRY FRUIT; HYDROGEN-PEROXIDE; BIOLOGICAL-CONTROL; SACCHAROMYCES-CEREVISIAE; POSTHARVEST DISEASES; CRYPTOCOCCUS-LAURENTII; PENICILLIUM-DIGITATUM; ANTIOXIDANT DEFENSE;
D O I：
10.1016/j.ijfoodmicro.2011.02.007
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The effect of H2O2-induced oxidative stress on the viability of the yeast antagonist. Cystofilobasidium infirmominiatum, as well as the effect of exogenous glycine betaine (GB) on yeast viability under oxidative stress, was determined. GB treatment improved the tolerance of C. infirmominiatum to oxidative stress. Compared to untreated control yeast cells, GB-treated cells showed less accumulation of reactive oxygen species (ROS) and a lower level of protein oxidation in response to oxidative stress. Additionally, GB-treated yeast exhibited greater biocontrol activity against Penicillium expansum and a faster growth in wounds of apple fruits stored at 25 degrees C compared to the performance of untreated yeast. The activities of antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPX) of C. infirmominiatum were elevated by GB treatment. Results indicate that the elicitation of antioxidant response by GB may contribute to improvements in oxidative stress tolerance, population growth in apple wounds, and biocontrol activity of C infirmominiatum. Published by Elsevier B.V.
引用
收藏
页码：76 / 83
页数：8
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