VvpM Induces Human Cell Death via Multifarious Modes Including Necroptosis and Autophagy

被引：8

作者：

Lee, Mi-Ae ^{[1
]}

Kim, Jeong-A ^{[1
]}

Shin, Mee-Young ^{[2
]}

Lee, Jeong K. ^{[1
]}

Park, Soon-Jung ^{[2
]}

Lee, Kyu-Ho ^{[1
]}

机构：

[1] Sogang Univ, Dept Life Sci, Seoul 121742, South Korea

[2] Yonsei Univ, Dept Environm Med Biol, Brain Korea PLUS Project Med Sci 21, Coll Med, Seoul 120752, South Korea

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2015年 / 25卷 / 02期

关键词：

Vibrio vulnificus; metalloprotease VvpM; necroptosis; autophagy; VIBRIO-VULNIFICUS; APOPTOSIS; LC3; PATHOGENESIS; ACTIVATION; REGULATOR; MARKER; P38;

D O I：

10.4014/jmb.1501.01007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

VvpM, one of the extracellular metalloproteases produced by Vibrio vulnificus, induces apoptotic cell death via a pathway consisting of ERK activation, cytochrome c release, and activation of caspases-9 and -3. VvpM-treated cells also showed necrotic cell death as stained by propidium iodide (PI). The percentage of PI-stained cells was decreased by pretreatment with Necrostatin-1, indicating that VvpM-mediated cell death occurs through necroptosis. The appearance of autophagic vesicles and lipidated form of light-chain-3B in rVvpM-treated cells suggests an involvement of autophagy in this process. Therefore, the multifarious action of VvpM might be one of the factors responsible for V. vulnificus pathogenesis.

引用

页码：302 / 306

页数：5

共 23 条

[1] Image Segmentation Method Using Thresholds Automatically Determined from Picture Contents
Chen, Yuan Been
Chen, Oscal T. -C.
[J]. EURASIP JOURNAL ON IMAGE AND VIDEO PROCESSING, 2009,
[2] Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury
Degterev A.
Huang Z.
Boyce M.
Li Y.
Jagtap P.
Mizushima N.
Cuny G.D.
Mitchison T.J.
Moskowitz M.A.
Yuan J.
[J]. Nature Chemical Biology, 2005, 1 (2) : 112 - 119
[3] Cell death by necrosis: towards a molecular definition
Golstein, Pierre
Kroemer, Guido
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 2007, 32 (01) : 37 - 43
[4] Identification of a Molecular Signaling Network that Regulates a Cellular Necrotic Cell Death Pathway
Hitomi, Junichi
Christofferson, Dana E.
Ng, Aylwin
Yao, Jianhua
Degterev, Alexei
Xavier, Ramnik J.
Yuan, Junying
[J]. CELL, 2008, 135 (07) : 1311 - 1323
[5] Construction and phenotypic evaluation of a Vibrio vulnificus vvpE mutant for elastolytic protease
Jeong, KC
Jeong, HS
Rhee, JH
Lee, SE
Chung, SS
Starks, AM
Escudero, GM
Gulig, PA
Choi, SH
[J]. INFECTION AND IMMUNITY, 2000, 68 (09) : 5096 - 5106
[6] LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
Kabeya, Y
Mizushima, N
Uero, T
Yamamoto, A
Kirisako, T
Noda, T
Kominami, E
Ohsumi, Y
Yoshimori, T
[J]. EMBO JOURNAL, 2000, 19 (21) : 5720 - 5728
[7] Vibrio vulnificus-induced death of Jurkat T-cells requires activation of p38 mitogen-activated protein kinase by NADPH oxidase-derived reactive oxygen species
Kim, Woo Hyang
Goo, Sung Young
Shin, Myeong Heon
Chun, Se-Jin
Lee, Heuiran
Lee, Kyu-Ho
Park, Soon-Jung
[J]. CELLULAR IMMUNOLOGY, 2008, 253 (1-2) : 81 - 91
[8] Vibrio vulnificus-induced Cell Death of Human Mononuclear Cells Requires ROS-dependent Activation of p38 and ERK 1/2 MAPKs
Kim, Woo Hyang
Goo, Sung Young
Lee, Kyu-Ho
Park, Soon-Jung
[J]. IMMUNOLOGICAL INVESTIGATIONS, 2009, 38 (01) : 31 - 48
[9] Classification of cell death: recommendations of the Nomenclature Committee on Cell Death
Kroemer, G
El-Deiry, W
Golstein, P
Peter, ME
Vaux, D
Vandenabeele, P
Zhivotovsky, B
Blagosklonny, MV
Malorni, W
Knight, RA
Piacentini, M
Nagata, S
Melino, G
[J]. CELL DEATH AND DIFFERENTIATION, 2005, 12 (Suppl 2) : 1463 - 1467
[10] Regulation of haemolysin (VvhA) production by ferric uptake regulator (Fur) in Vibrio vulnificus: repression of vvhA transcription by Fur and proteolysis of VvhA by Fur-repressive exoproteases
Lee, Hyun-Jung
Kim, Jeong-A
Lee, Mi-Ae
Park, Soon-Jung
Lee, Kyu-Ho
[J]. MOLECULAR MICROBIOLOGY, 2013, 88 (04) : 813 - 826

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