CRKL protein overexpression enhances cell proliferation and invasion in pancreatic cancer

被引：15

作者：

Fu, Lin ^{[1
,2
]}

Dong, Qianze ^{[1
,2
]}

Xie, Chengyao ^{[1
,2
]}

Wang, Yan ^{[1
,2
]}

Li, Qingchang ^{[1
,2
]}

机构：

[1] China Med Univ, Affiliated Hosp 1, Dept Pathol, Shenyang 11000, Liaoning, Peoples R China

[2] China Med Univ, Coll Basic Med Sci, Shenyang 11000, Liaoning, Peoples R China

来源：

TUMOR BIOLOGY | 2015年 / 36卷 / 02期

关键词：

CRKL; Proliferation; Invasion; Pancreatic cancer; ADAPTER; MMP-2; INDUCTION;

D O I：

10.1007/s13277-014-2706-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

CRKL is an adapter protein which is overexpressed in many malignant tumors and plays crucial roles in tumor progression. However, expression pattern and biological roles of CRKL in pancreatic cancer have not been examined. In the present study, we found that CRKL expression in pancreatic cancer specimens was higher than that in normal pancreatic tissues. Colony formation assay and Matrigel invasion assay showed that the overexpression of CRKL in Bxpc3 and Capan2 cell lines with low endogenous expression increased cell proliferation and invasion. Flow cytometry showed that CRKL promoted cell proliferation by facilitating cell cycle. Further analysis of cell cycle- and invasion-related molecules showed that CRKL upregulated cyclin D1, cyclin A, matrix metalloproteinase 2 (MMP2) expression, and phosphorylated extracellular signal (ERK)-regulated kinase. In conclusion, our study demonstrated that CRKL was overexpressed in human pancreatic cancers and contributed to pancreatic cancer cell proliferation and invasion through ERK signaling.

引用

页码：1015 / 1022

页数：8

共 32 条

[31] Signaling pathways in Ras-mediated tumorigenicity and metastasis [J].