Comparing protein abundance and mRNA expression levels on a genomic scale

被引：1374
作者：
Greenbaum, D
Colangelo, C
Williams, K
Gerstein, M
机构：
[1] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
[2] Yale Univ, Dept Genet, New Haven, CT 06520 USA
[3] Yale Univ, Biopolymer Lab, HHMI, New Haven, CT 06520 USA
[4] Yale Univ, WM Keck Fdn, Biotechnol Resource Lab, New Haven, CT 06520 USA
[5] Yale Univ, Dept Comp Sci, New Haven, CT 06520 USA
来源：
GENOME BIOLOGY | 2003年 / 4卷 / 09期
关键词：
D O I：
10.1186/gb-2003-4-9-117
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Attempts to correlate protein abundance with mRNA expression levels have had variable success. We review the results of these comparisons, focusing on yeast. In the process, we survey experimental techniques for determining protein abundance, principally two-dimensional gel electrophoresis and mass-spectrometry. We also merge many of the available yeast protein-abundance datasets, using the resulting larger 'meta-dataset' to find correlations between protein and mRNA expression, both globally and within smaller categories.
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页数：8
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