Characterization of a thermo-adapted strain of Zymomonas mobilis for ethanol production at high temperature

被引：11

作者：

Samappito, Jatupat ^{[1
]}

Yamada, Mamoru ^{[2
]}

Klanrit, Preekamol ^{[1
,3
]}

Thanonkeo, Pornthap ^{[1
,3
]}

机构：

[1] Khon Kaen Univ, Fac Technol, Dept Biotechnol, Khon Kaen 40002, Thailand

[2] Yamaguchi Univ, Dept Biol Chem, Fac Agr, Yamaguchi 7538515, Japan

[3] Khon Kaen Univ, Fermentat Res Ctr Value Added Agr Prod, Khon Kaen 40002, Thailand

来源：

3 BIOTECH | 2018年 / 8卷 / 11期

关键词：

Ethanol production; High-temperature fermentation; Thermal stress adaptation; Thermotolerance; Zymomonas mobilis; EVOLUTION;

D O I：

10.1007/s13205-018-1493-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A thermo-adapted strain of Zymomonas mobilis designated ZM AD41 that capable of growth and ethanol production at high temperature was obtained using the thermal stress adaptation technique. This thermo-adapted strain exhibited approximately 1.8- and 27-fold higher growth rate than the wild-type at 39 degrees C and 41 degrees C, respectively. It was more resistant to stress induced by acetic acid at 200mM and hydrogen peroxide (H2O2) at 0.4mM and produced approximately 1.8- and 38.6-fold higher ethanol concentrations than the wild-type at 39 degrees C and 41 degrees C, respectively. Moreover, it had better sedimentation performance during ethanol fermentation at high temperature than the wild-type. Based on the growth performance, heat, acetic acid and H2O2 stress treatments, sedimentation characteristics, and ethanol fermentation capability, Z. mobilis ZM AD41 was a good candidate for ethanol production at high temperature.

引用

页数：9

共 20 条

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