A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and γ-ray-irradiation, and freezing

被引：16

作者：

Noda, Masami ^{[1
]}

Ma, Yue ^{[1
]}

Yoshikawa, Yuko ^{[1
]}

Imanaka, Tadayuki ^{[2
]}

Mori, Toshiaki ^{[3
]}

Furuta, Masakazu ^{[3
]}

Tsuruyama, Tatsuaki ^{[4
]}

Yoshikawa, Kenichi ^{[1
]}

机构：

[1] Doshisha Univ, Fac Life & Med Sci, Kyoto 6100321, Japan

[2] Ritsumeikan Univ, Res Org Sci & Technol, Shiga 5258577, Japan

[3] Osaka Prefecture Univ, Grad Sch Engn, Dept Quantum & Radiat Technol, Osaka 5998570, Japan

[4] Kyoto Univ, Grad Sch Med, Dept Pathol, Drug Discovery & Med, Kyoto 6068501, Japan

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

日本学术振兴会;

关键词：

DIMETHYL-SULFOXIDE; ASCORBIC-ACID; GIANT DNA; DAMAGE; DIMETHYLSULFOXIDE; CRYOPRESERVATION; RADIOPROTECTION; COMPACTION; MECHANISM; DEATH;

D O I：

10.1038/s41598-017-08894-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Dimethyl sulfoxide (DMSO) is widely used as a cryoprotectant for organs, tissues, and cell suspension in storage. In addition, DMSO is known to be a useful free radical scavenger and a radio-protectant. To date, many in vitro assays using cultured cells have been performed for analysing the protective effect of DMSO against genomic DNA damage; however, currently it has been rather difficult to detect DNA double strand breaks (DSBs) in a quantitative manner. In the present study, we aimed to observe the extent of DNA damage by use of single molecular observation with a fluorescence microscope to evaluate DSBs induced by photo- and gamma-ray-irradiation, or freeze/thawing in variable concentrations of DMSO. As a result, we found that 2% DMSO conferred the maximum protective effect against all of the injury sources tested, and these effects were maintained at higher concentrations. Further, DMSO showed a significantly higher protective effect against freezing-induced damage than against photoand gamma-ray-irradiation-induced damage. Our study provides significant data for the optimization of DNA cryopreservation with DMSO, as well as for the usage of DNA as the protective agent against the injuries caused by active oxygen and radiations.

引用

页数：8

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