Novel Folate Binding Protein in Arabidopsis Expressed during Salicylic Acid-Induced Folate Accumulation

被引：7

作者：

Puthusseri, Bijesh ^{[1
]}

Divya, Peethambaran ^{[1
,3
]}

Lokesh, Veeresh ^{[1
]}

Kumar, Gyanendra ^{[1
]}

Savanur, Mohammed Azharuddin ^{[2
,3
]}

Neelwarne, Bhagyalakshmi ^{[1
]}

机构：

[1] CSIR, Cent Food Technol Res Inst, Plant Cell Biotechnol Dept, Mysore 570020, Karnataka, India

[2] Karnatak Univ, Dept Biochem, Dharwad 580003, Karnataka, India

[3] Indian Inst Sci, Bangalore 560012, Karnataka, India

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2018年 / 66卷 / 02期

关键词：

folate binding protein; folate receptor; At5G27830; plant folate; metabolic engineering; FOLIC-ACID; BIOFORTIFICATION; STABILITY; PLANTS; RICE;

D O I：

10.1021/acs.jafc.7b04236

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Increasing the quantity of natural folates implant foods is recently gaining significant interest, owing to their acute deficiencies in various populations. This study observed that foliar salicylic acid treatment enhanced the accumulation of folates in Arabidopsis, which correlated with the increase in a folate binding protein (FBP) and the expression of mRNA of a putative folate binding protein AtSG27830. A protein band corresponding to similar to 43 kDa was observed after resolving the affinity purified protein on SDS-PAGE, and the partial amino acid sequence indicated that the protein is indeed At5G27830 Docking studies performed with At5G27830 confirmed specific binding of folic acid to predicted site. Heterologous expression of At5G27830 in the,yeast resulted insignificant uptake and accumulation of folic acid in cells. This novel study of a plant FBP will be useful for folate metabolic engineering of a wide range of crops.

引用

页码：505 / 511

页数：7

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