Expression of β-(1,3-1,4)-glucanase gene of Orpinomyces sp GMLF18 in Escherichia coli EC1000 and Lactococcus lactis subsp cremoris MG1363

被引：5

作者：

Comlekcioglu, Ugur ^{[1
]}

Ozkose, Emin ^{[1
]}

Akyol, Ismail ^{[1
]}

Yazdic, Ferit Can ^{[1
]}

Ekinci, Mehmet Sait ^{[1
]}

机构：

[1] Kahramanmaras Sutcu Imam Univ, Fac Agr, Dept Anim Sci, Biotechnol & Gene Engn Lab, Kahramanmaras, Turkey

来源：

TURKISH JOURNAL OF BIOLOGY | 2011年 / 35卷 / 04期

关键词：

Orpthomyces; beta-(1,3-1,4)-glucanase; licA; Lactococcus lactis; lichenan; LACTOBACILLUS-REUTERI; STREPTOCOCCUS-LACTIS; MOLECULAR-CLONING; BETA-GLUCANASE; CEREAL-GRAINS; RUMINAL FUNGI; SEQUENCE; XYLANASE; CELLULASES; DEGRADATION;

D O I：

10.3906/biy-0912-25

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A gene encoding beta-(1,3-1,4)-glucanase (licA) was amplified from Orpinomyces sp. GMLF18 and expressed in Escherichia colt. The DNA sequence of licA showed that the gene was 707 bp and encoded a protein with a molecular mass of 26 kDa that belongs to family glycosyl hydrolase 16. The main LicA activity was observed to be cell-associated for the licA containing transformant E. coli, and the enzyme expressed in E. coli showed the highest activity at pH 5.0-6.0 and at temperatures of 40-50 degrees C. The enzyme was found to be stable at 40 degrees C; however, 12% of LicA activity was lost at 50 degrees C in 20 min. The licA was then introduced into the facultative anaerobic bacterium Lactococcus lactis subsp. cremoris MG1363 by a stable recombinant plasmid, pIL253. Although the enzymatic activity was lower than that in E. coli, the gene encoding the fungal originated lichenase was successfully expressed in L. lactis.

引用

页码：405 / 414

页数：10