Identification of 67 Histone Marks and Histone Lysine Crotonylation as a New Type of Histone Modification

被引：1414

作者：

Tan, Minjia ^{[1
]}

Luo, Hao ^{[1
]}

Lee, Sangkyu ^{[1
]}

Jin, Fulai ^{[2
,3
]}

Yang, Jeong Soo ^{[1
]}

Montellier, Emilie ^{[6
,7
,8
]}

Buchou, Thierry ^{[6
,7
,8
]}

Cheng, Zhongyi ^{[1
]}

Rousseaux, Sophie ^{[6
,7
,8
]}

Rajagopal, Nisha ^{[2
,3
]}

Lu, Zhike ^{[1
]}

Ye, Zhen ^{[2
,3
]}

Zhu, Qin ^{[4
]}

Wysocka, Joanna ^{[5
]}

Ye, Yang ^{[4
]}

Khochbin, Saadi ^{[6
,7
,8
]}

Ren, Bing ^{[2
,3
]}

Zhao, Yingming ^{[1
]}

机构：

[1] Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA

[2] Univ Calif San Diego, Ludwig Inst Canc Res, Sch Med, La Jolla, CA 92093 USA

[3] Univ Calif San Diego, Dept Cellular & Mol Med, Sch Med, La Jolla, CA 92093 USA

[4] Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China

[5] Stanford Univ, Dept Chem & Syst Biol, Sch Med, Stanford, CA 94305 USA

[6] INSERM, U823, F-38706 La Tronche, France

[7] Univ Grenoble 1, F-38706 La Tronche, France

[8] Fac Med, Inst Albert Bonniot, F-38706 La Tronche, France

来源：

CELL | 2011年 / 146卷 / 06期

关键词：

MASS-SPECTROMETRY; POSTTRANSLATIONAL MODIFICATIONS; CHROMATIN MODIFICATIONS; HUMAN-CELLS; CODE; DISTINCT; H3; PHOSPHORYLATION; ACETYLATION; METHYLATION;

D O I：

10.1016/j.cell.2011.08.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the identification of 67 previously undescribed histone modifications, increasing the current number of known histone marks by about 70%. We further investigated one of the marks, lysine crotonylation (Kcr), confirming that it represents an evolutionarily-conserved histone posttranslational modification. The unique structure and genomic localization of histone Kcr suggest that it is mechanistically and functionally different from histone lysine acetylation (Kac). Specifically, in both human somatic and mouse male germ cell genomes, histone Kcr marks either active promoters or potential enhancers. In male germinal cells immediately following meiosis, Kcr is enriched on sex chromosomes and specifically marks testis-specific genes, including a significant proportion of X-linked genes that escape sex chromosome inactivation in haploid cells. These results therefore dramatically extend the repertoire of histone PTM sites and designate Kcr as a specific mark of active sex chromosome-linked genes in postmeiotic male germ cells.

引用

页码：1015 / 1027

页数：13

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