Using liquid crystals as a readout system in urinary albumin assays

被引：62
作者：
Alino, Vera Joanne [1 ]
Yang, Kun-Lin [1 ]
机构：
[1] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117576, Singapore
来源：
ANALYST | 2011年 / 136卷 / 16期
关键词：
NANOMETER-SCALE TOPOGRAPHY; PESTICIDE DETECTION; SURFACES; PROTEIN; MICROALBUMINURIA; QUANTIFICATION; IMMUNOSENSORS; AMPLIFICATION; IMMUNOASSAYS; FEASIBILITY;
D O I：
10.1039/c1an15143f
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Detecting human serum albumin (HSA) in urine samples is a standard procedure for the diagnosis of kidney problems and the prognosis of patients with chronic kidney diseases (CKDs). In this study, we developed a HSA assay by incorporating a thin layer of liquid crystals (LCs) as a readout system such that the presence of HSA in urine samples can be detected as optical signals. In combination with dilution protocols, this assay can be used to estimate the concentration range of HSA simply by counting the number of bright spots. Our results show that the assay can detect HSA at concentrations as low as 15 mu g mL(-1). It is anticipated that this assay can provide a faster and simpler alternative for the diagnosis and prognosis of patients with kidney diseases.
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收藏
页码：3307 / 3313
页数：7
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