Gold nanoparticle modified conducting polymer of 4-(2,5-di(thiophen-2-yl)-1H-pyrrole-1-l) benzenamine for potential use as a biosensing material

被引：40

作者：

Tuncagil, Sevinc ^{[2
,3
,4
]}

Ozdemir, Caglar ^{[1
]}

Demirkol, Dilek Odaci ^{[1
]}

Timur, Suna ^{[1
]}

Toppare, Levent ^{[2
,3
,4
]}

机构：

[1] Ege Univ, Dept Biochem, Fac Sci, TR-35100 Bornova, Turkey

[2] Middle E Tech Univ, Dept Chem, TR-06531 Ankara, Turkey

[3] Middle E Tech Univ, Div Biotechnol, TR-06531 Ankara, Turkey

[4] Middle E Tech Univ, Dept Biotechnol, TR-06531 Ankara, Turkey

来源：

FOOD CHEMISTRY | 2011年 / 127卷 / 03期

关键词：

Electrochemical polymerisation; Electrochemical biosensors; Conducting polymers; Gold nanoparticles (AuNPs); FLOW-INJECTION ANALYSIS; GLUCOSE-OXIDASE; DIRECT ELECTROCHEMISTRY; HIGH-SENSITIVITY; IMMOBILIZATION; ELECTRODES; INVERTASE; MATRIX; SENSOR; FILM;

D O I：

10.1016/j.foodchem.2011.01.089

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Gold nanoparticle (AuNP) modified conducting polymer of 4-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine (SNS-NH2) was used as the biosensing platform for glucose analysis. Electrochemical measurements were carried out by following the consumed oxygen due to the enzymatic reaction of glucose oxidase (GOx) at -0.7 V vs Ag/AgCl. Optimisation of pH, enzyme loading, stability experiments were carried out. Effect of NP was investigated by monitoring the signal responses at different AuNP sizes and amounts. A linear relation of y = 1.597x + 0.264 (R-2 = 0.993) was found for glucose concentrations between 0.002 and 5.0 mM. The analytical characteristics of the system were also evaluated for glucose determination in flow injection analysis (FIA) mode. Finally, the system was checked for glucose detection on real samples. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1317 / 1322

页数：6

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