Evaluation of a silicon oxynitride hydrophilic interaction liquid chromatography column in saccharide and glycoside separations

被引：14
作者：
Wan, Huihui [1 ]
Sheng, Qianying [2 ]
Zhong, Hongmin [1 ]
Guo, Xiujie [3 ]
Fu, Qing [2 ]
Liu, Yanfang [3 ]
Xue, Xingya [3 ]
Liang, Xinmiao [3 ]
机构：
[1] Dalian Univ Technol, Analyt Ctr, Fac Chem Environm & Biol Sci & Technol, Dalian 116024, Peoples R China
[2] E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China
[3] Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
来源：
JOURNAL OF SEPARATION SCIENCE | 2015年 / 38卷 / 08期
关键词：
Carbohydrates; Hydrophilic interaction liquid chromatography; Silica; Silicon oxynitride; Stationary phases; ANION-EXCHANGE CHROMATOGRAPHY; STATIONARY PHASES; MASS-SPECTROMETRY; POLAR COMPOUNDS; PERFORMANCE; SELECTIVITY; RETENTION; SURFACE; METABOLOMICS; PEPTIDES;
D O I：
10.1002/jssc.201401413
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The retention characteristics of a silicon oxynitride stationary phase for carbohydrate separation were studied in hydrophilic interaction chromatography mode. Four saccharides including mono-, di-, and trisaccharides were employed to investigate the effects of water content and buffer concentration in the mobile phase on hydrophilic interaction liquid chromatography retention. For the tested saccharides, the silicon oxynitride column demonstrated excellent performance in terms of separation efficiency, hydrophilicity, and interesting separation selectivity for carbohydrates compared to the bare silica stationary phase. Finally, the silicon oxynitride hydrophilic interaction liquid chromatography column was employed in the separation of complex samples of fructooligosaccharides, saponins, and steviol glycoside from natural products. The resulting chromatograms demonstrated good separation efficiency and longer retention compared with silica, which further confirmed the advantages and potential application of silicon oxynitride stationary phase for hydrophilic interaction liquid chromatography separation.
引用
收藏
页码：1271 / 1276
页数：6
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