Enhanced formation of hydroxyapatites in gelatin/imogolite macroporous hydrogels

Hypothesis: Gelatin is widely investigated for the fabrication of synthetic scaffolds in bone tissue engineering. Practical limitations to its use are mainly due to the fast dissolution rate in physiological conditions and to the lack of pores with suitable dimensions for cell permeation. The aim of this work is to exploit imogolite clays as nucleation sites for the growth of calcium phosphates in gelatin-based hydro gels and to take advantage of a cryogenic treatment to obtain pores of similar to 100 mu m. Experiments: We evaluated the effect of imogolites and a biocompatible cross-linker on the gelatin network in terms of morphology, thermal and rheological behavior. The hydrogels were cryogenically treated and characterized to investigate the modification of the polymer network, both at the micro and nano-scale. The samples were mineralized to investigate the effect of imogolites on the formation of calcium phosphates. Findings: The interaction between gelatin, imogolite and cross-linker leads to the modification of the hydrogel structure at the micro-scale, while minor effects are detected at the nano-scale. The cryogenic procedure is successful in generating pores with the desired size, while the presence of imogolites in the hydrogel promotes hydroxyapatites formation. These results demonstrate that imogolites can be effectively employed as functional fillers in polymer-based scaffolds. (C) 2017 Elsevier Inc. All rights reserved.