Construction of an infectious full-length cDNA clone of potato virus M

被引：22
|
作者：
Flatken, S. [1 ]
Ungewickell, V. [1 ]
Menzel, W. [1 ]
Maiss, E. [1 ]
机构：
[1] Leibniz Univ Hannover, Inst Plant Dis & Plant Protect, D-30419 Hannover, Germany
来源：
ARCHIVES OF VIROLOGY | 2008年 / 153卷 / 07期
关键词：
D O I：
10.1007/s00705-008-0127-5
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
An infectious full-length cDNA clone of potato virus M (PVM) was produced. Total RNA was extracted from PVM-infected Nicotiana hesperis plants and used for cDNA synthesis. Subsequent RT-PCR produced two DNA fragments of about 5.5 and 3.2 kbp, which were ligated downstream of an enhanced 35S cauliflower mosaic virus promoter. After cloning of the enhanced 35S promoter with the PVM sequence into a modified pBIN19 plasmid and electroporation of Agrobacterium tumefaciens, the agroinoculated PVM full-length clone (pPVM-flc) led to systemic PVM infections in different host plants, causing symptoms indistinguishable from those caused by wild-type PVM.
引用
收藏
页码：1385 / 1389
页数：5
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