Monitoring Wound Healing with Topically Applied Optical NanoFlare mRNA Nanosensors

被引：20

作者：

Hwang, Jangsun ^{[1
,2
]}

Seo, Youngmin ^{[3
,4
]}

Jeong, Daun ^{[2
]}

Ning, Xiaoyu ^{[1
,5
]}

Wiraja, Christian ^{[1
]}

Yang, Lixia ^{[1
]}

Tan, Chew Teng ^{[6
]}

Lee, Jinhyuck ^{[2
]}

Kim, Yesol ^{[7
]}

Kim, Ji Won ^{[7
]}

Kim, Dai Hyun ^{[8
]}

Choi, Jonghoon ^{[7
]}

Lim, Chin Yan ^{[6
,9
]}

Pu, Kanyi ^{[1
]}

Jang, Woo Young ^{[2
]}

Xu, Chenjie ^{[10
]}

机构：

[1] Nanyang Technol Univ, Sch Chem & Biomed Engn, 62 Nanyang Dr, Singapore 637457, Singapore

[2] Korea Univ, Dept Orthoped Surg, Coll Med, 73 Korea Ro, Seoul 02841, South Korea

[3] Yonsei Univ, Sch Elect & Elect Engn, 50 Yonsei Ro, Seoul 03722, South Korea

[4] OID Ltd, Dept Res & Dev, 249-2,123 Osongsaengmyeong Ro, Cheongju 28160, Chungcheongbuk, South Korea

[5] Nanyang Technol Univ, NTU Inst Hlth Technol, Interdisciplinary Grad Sch, 61 Nanyang Dr, Singapore 637335, Singapore

[6] Agcy Sci Technol & Res, A STAR Skin Res Labs, 8A Biomed Grove, Singapore 138648, Singapore

[7] Chung Ang Univ, Sch Integrat Engn, 84 Heukseok Ro, Seoul 06974, South Korea

[8] Korea Univ, Dept Dermatol, Coll Med, 73 Korea Ro, Seoul 02841, South Korea

[9] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Biochem, MD 7,8 Med Dr, Singapore 117596, Singapore

[10] City Univ Hong Kong, Dept Biomed Engn, Kowloon, 83 Tat Chee Ave, Hong Kong, Peoples R China

来源：

ADVANCED SCIENCE | 2022年 / 9卷 / 18期

关键词：

diabetic wound; mRNA nanosensors; NanoFlare; spherical nucleic acids; wound healing; ENDOTHELIAL GROWTH-FACTOR; SPHERICAL NUCLEIC-ACIDS; BIOMARKERS; FIBROBLAST; IDENTIFICATION; NANOPARTICLES; PECAM-1; MARKER;

D O I：

10.1002/advs.202104835

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An effective wound management strategy needs accurate assessment of wound status throughout the whole healing process. This can be achieved by examining molecular biomarkers including proteins, DNAs, and RNAs. However, existing methods for quantifying these biomarkers such as immunohistochemistry and quantitative polymerase chain reaction are usually laborious, resource-intensive, and disruptive. This article reports the development and utilization of mRNA nanosensors (i.e., NanoFlare) that are topically applied on cutaneous wounds to reveal the healing status through targeted and semi-quantitative examination of the mRNA biomarkers in skin cells. In 2D and 3D in vitro models, the efficacy and efficiency of these nanosensors are demonstrated in revealing the dynamic changes of mRNA biomarkers for different stages of wound development. In mouse models, this platform permits the tracking and identification of wound healing stages and a normal and diabetic wound healing process by wound healing index in real time.

引用

页数：14

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[1] VASCULAR ENDOTHELIAL GROWTH-FACTOR IN OCULAR FLUID OF PATIENTS WITH DIABETIC-RETINOPATHY AND OTHER RETINAL DISORDERS [J].