Synthesis of immobilized CMP-sialic acids and their enzymatic transfer with sialyltransferase

被引：0
作者：
Kajihara, Y
Ebata, T
Kodama, H
机构：
[1] Yokohama City Univ, Fac Sci, Dept Syst Funct, Kanazawa Ku, Yokohama, Kanagawa 2360027, Japan
[2] Japan Tobacco Inc, Life Sci Res Lab, Aoba Ku, Yokohama, Kanagawa 2270052, Japan
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 1998年 / 37卷 / 22期
关键词：
enzymes; glycoproteins; immobilization; sialic acids; solid-phase synthesis;
D O I：
10.1002/(SICI)1521-3773(19981204)37:22<3166::AID-ANIE3166>3.0.CO;2-I
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel immobilization procedure for glycoproteins is based on the reaction of immobilized CMP-NeuAc (CMP-sialic acids) with sialyltransferase (see scheme). The transfer ability was tested under various conditions; a corresponding oligosaccharide or asialoglycoprotein acted as sialyl acceptor. The solid-phase synthesis of CMP-NeuAc is also described.
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页码：3166 / 3169
页数：4
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