Synthesis of immobilized CMP-sialic acids and their enzymatic transfer with sialyltransferase

被引：0

作者：

Kajihara, Y

Ebata, T

Kodama, H

机构：

[1] Yokohama City Univ, Fac Sci, Dept Syst Funct, Kanazawa Ku, Yokohama, Kanagawa 2360027, Japan

[2] Japan Tobacco Inc, Life Sci Res Lab, Aoba Ku, Yokohama, Kanagawa 2270052, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 1998年 / 37卷 / 22期

关键词：

enzymes; glycoproteins; immobilization; sialic acids; solid-phase synthesis;

D O I：

10.1002/(SICI)1521-3773(19981204)37:22<3166::AID-ANIE3166>3.0.CO;2-I

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel immobilization procedure for glycoproteins is based on the reaction of immobilized CMP-NeuAc (CMP-sialic acids) with sialyltransferase (see scheme). The transfer ability was tested under various conditions; a corresponding oligosaccharide or asialoglycoprotein acted as sialyl acceptor. The solid-phase synthesis of CMP-NeuAc is also described.

引用

收藏

页码：3166 / 3169

页数：4

相关论文

共 14 条

[1] Glycobiology: Toward understanding the function of sugars [J].

Dwek, RA .

CHEMICAL REVIEWS, 1996, 96 (02) :683-720

[2] HIGH-RESOLUTION H-1-NMR IN SOLID-PHASE ORGANIC-SYNTHESIS [J].

FITCH, WL ;

DETRE, G ;

HOLMES, CP ;

SHOOLERY, JN ;

KEIFER, PA .

JOURNAL OF ORGANIC CHEMISTRY, 1994, 59 (26) :7955-7956

[3] ENZYMATIC INTRODUCTION OF A FLUORESCENT SIALIC-ACID INTO OLIGOSACCHARIDE CHAINS OF GLYCOPROTEINS [J].

GROSS, HJ ;

BROSSMER, R .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 177 (03) :583-589

[4]

JOURDIAN GW, 1971, J BIOL CHEM, V246, P430

[5] EFFICIENT CHEMICAL SYNTHESIS OF CMP-NEU5AC AND CMP-(NEU5AC-ALPHA-2-]-8NEU5AC) [J].

KAJIHARA, Y ;

EBATA, T ;

KOSEKI, K ;

KODAMA, H ;

MATSUSHITA, H ;

HASHIMOTO, H .

JOURNAL OF ORGANIC CHEMISTRY, 1995, 60 (17) :5732-5735

[6] ENZYMATIC TRANSFER OF 6-MODIFIED D-GALACTOSYL RESIDUES - SYNTHESIS OF BIANTENNARY PENTA-SACCHARIDES AND HEPTA-SACCHARIDES HAVING 2 6-DEOXY-D-GALACTOSE RESIDUES AT THE NONREDUCING END AND EVALUATION OF 6-DEOXY-D-GALACTOSYL TRANSFER TO GLYCOPROTEIN USING BOVINE BETA-(1-]4)-GALACTOSYLTRANSFERASE AND UDP-6-DEOXY-D-GALACTOSE [J].

KAJIHARA, Y ;

ENDO, T ;

OGASAWARA, H ;

KODAMA, H ;

HASHIMOTO, H .

CARBOHYDRATE RESEARCH, 1995, 269 (02) :273-294

[7] CHARACTERIZATION OF INHIBITORY ACTIVITIES AND BINDING MODE OF SYNTHETIC 6'-MODIFIED METHYL N-ACETYL-BETA-LACTOSAMINIDE TOWARD RAT-LIVER CMP-DEUTERIUM-NEU5ACDEUTERIUM-GALACTOSIDE-(2-]6)-ALPHA-DEUTERIUM-SIALYLTRANSFERASE [J].

KAJIHARA, Y ;

KODAMA, H ;

WAKABAYASHI, T ;

SATO, K ;

HASHIMOTO, H .

CARBOHYDRATE RESEARCH, 1993, 247 :179-193

[8] STEREOSELECTIVE SYNTHESIS OF 2-THIOGLYCOSIDES OF N-ACETYLNEURAMINIC ACID [J].

MARRA, A ;

SINAY, P .

CARBOHYDRATE RESEARCH, 1989, 187 (01) :35-42

[9]

MOSBACH K, 1976, METHOD ENZYMOL, V44, P134

[10] SYNTHESIS AND ENZYMATIC AND NMR-STUDIES OF NOVEL SIALOSIDE PROBES - UNPRECEDENTED, SELECTIVE NEURAMINIDASE HYDROLYSIS OF AND INHIBITION BY C-6-(METHYL)-GAL SIALOSIDES [J].

SABESAN, S ;

NEIRA, S ;

DAVIDSON, F ;

DUUS, JO ;

BOCK, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (05) :1616-1634