Site-Specific Attachment of Proteins onto a 3D DNA Tetrahedron through Backbone-Modified Phosphorothioate DNA

被引：40
|
作者：
Wong, Ngo Yin
Zhang, Chuan [2 ]
Tan, Li Huey [1 ]
Lu, Yi [1 ]
机构：
[1] Univ Illinois, Beckman Inst Adv Sci & Technol, Dept Chem, Urbana, IL 61801 USA
[2] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
来源：
SMALL | 2011年 / 7卷 / 10期
基金：
美国国家科学基金会;
关键词：
GOLD NANOPARTICLE ARRAYS; NANOSCALE SHAPES; FOLDING DNA; NANOSTRUCTURES; MOLECULES; POLYHEDRA; LINKERS;
D O I：
10.1002/smll.201100140
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Phosphorothioate-modified DNA and short bifunctional linkers are used to attach proteins to a DNA tetrahedron. Precise control of the position of and distance between protein modifications is achieved. This technique is simple, can be adapted to other DNA nanostructures, exhibits high yields, and the modification can be programmably placed within the structure. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：1427 / 1430
页数：4
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